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<h4>Background</h4>Metabolic reprogramming and the formation of an immunosuppressive tumor microenvironment(TME) are hallmarks of osteosarcoma (OS). However, the metabolic characteristics of OS and its associated immune microenvironment remain largely unknown.<h4>Methods</h4>The single-cell data were processed for dimensionality …
Read PDF<h4>Background</h4>Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy with complex genetic underpinnings. Understanding the causal relationships between genetic factors and AML risk is crucial for developing targeted therapeutic strategies.<h4>Methods</h4>We conducted a comprehensive Mendelian randomization …
Read PDFThe common octopus, Octopus vulgaris, is renowned for its advanced nervous system and complex behavior, yet its immune system remains poorly understood despite its relevance for health and pathogen resistance. Understanding octopus immunity is key …
Read PDFOligodendrocytes produce myelin, a lipid-rich membrane that wraps neuronal axons in the central nervous system to provide metabolic and trophic support and allow for saltatory conduction. Developation requires precisely timed and localized neuron-oligodendrocyte communication. In …
Read PDF<h4>Background</h4>Ischemic stroke (IS) is a severe neurological disorder, with inflammation playing a crucial role in its development. This study is aimed at investigating the gene expression profiles related to inflammation in IS patients and determining …
Read PDFFungal pathogens represent a rising global concern with increasing impacts on human health and food security. Despite their significance, research on fungal infections continues to lag behind other infectious diseases, hindering diagnostic and treatment advances. …
Read PDFSingle cell assay for transposase-accessible chromatin using sequencing (scATAC-seq) is a technology that allows for analysis of chromatin accessibility at a single cell resolution. scATAC-seq is advancing our understanding of gene regulation, and has found …
Read PDFPopulation-level immune single-cell profiling has the potential to enable the association of cellular function with human ancestry, demographic, and environmental variables. However, methods for collecting and processing peripheral blood mononuclear cells (PBMCs) for single-cell sequencing …
Read PDFIschemic stroke represents a significant global health hazard, causing substantial morbidity, mortality, and long-term disability worldwide. The brain's complexity necessitates targeting ischemic stroke dysfunction syndrome. Single-cell RNA sequencing (scRNA-seq) offers unbiased, high-resolution analysis of cell …
Read PDF<h4>Background</h4>Cervical cancer is among the most prevalent gynecological malignancies globally, and its inherent molecular heterogeneity remains a persistent obstacle to uniform treatment efficacy and patient survival. Lysosome-mediated cell death (LDCD) has recently emerged as a …
Read PDF<h4>Background</h4>Mucosal-associated invariant T (MAIT) cells are innate-like T lymphocytes involved in immune surveillance and tissue homeostasis. Yet, their transcriptional dynamics and functional crosstalk with macrophages at the time of myocardial injury are incompletely defined.<h4>Methods</h4>We used …
Read PDFUnderstanding chromatin dynamics across the cell cycle is crucial, as the structural transitions of chromosomes are fundamental to processes including transcriptional regulation, DNA replication, and faithful chromosome segregation. Although chromatin undergoes extensive reorganization throughout the …
Read PDFDrought stress is the most pervasive abiotic constraint on global crop productivity, with projected intensification under climate change threatening the yields of staple crops including wheat, rice, maize, and legumes. Conventional breeding approaches have delivered …
Read PDFVirus-host interactions govern infection outcomes and viral evolution, but host determinants that enable or restrict viral replication have been difficult to map comprehensively and in the right cellular contexts. Pooled CRISPR perturbation screens now provide …
Read PDF<h4>Background</h4>Airway hyperresponsiveness (AHR) is a clinically important feature of chronic pediatric inflammatory airway disease, but its cellular architecture remains incompletely resolved. Because bulk transcriptomic approaches can mask signals from rare or state-specific cell populations, single-cell …
Read PDFHere, we present a protocol for mapping cell-type-specific cis-acting expression quantitative trait loci (cis-eQTLs) in a target tissue and integrating them with genome-wide association study (GWAS) data. We describe pooled-sample multiplexed sequencing, genotype-based cell demultiplexing, …
Read PDFThis review aims to provide a comprehensive overview of recent advances in the genomics of multiple myeloma and their clinical implications. Multiple myeloma is a hematologic malignancy originating from bone marrow plasma cells, characterized by …
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Read PDFBACKGROUND: Pig is an important model for biomedical studies, yet the immune cell heterogeneity, the subtle relationship between immune cells and the inter-breed immune variation in pigs remains poorly characterized. Here, we generate single-cell transcriptomes …
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