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While single-cell RNA sequencing has advanced our understanding of cell fate, identifying molecular hallmarks of potency-a cell's ability to differentiate into other cell types-remains a challenge. Here we introduce CytoTRACE 2, an interpretable deep learning …
Read PDF<h4>Background</h4>The high dimensionality of data in single cell transcriptomics (scRNAseq) requires investigators to choose subsets of genes ("feature selection") for downstream analysis (e.g., unsupervised cell clustering). The evaluation of different approaches to feature selection is …
Read PDFThe tonsil is a highly compartmentalized organ in which different microanatomical structures orchestrate designated (immune) functions. We use this already well-studied tissue to survey imaging-based spatial transcriptomics data for studying immune responses in native tissue …
Read PDFPulmonary fibrosis represents a progressive interstitial lung disease marked by excessive extracellular matrix deposition and architectural distortion. Vascular endothelial cells critically contribute to fibrogenesis through paracrine secretion of pro-fibrotic mediators, yet their mechanobiological regulation remains …
Read PDFInsect wing development involves tissue patterning, cell fate transitions, and hormone signaling, yet its spatiotemporal logic remains unclear. The silkworm, with large wing discs and defined stages, provides an ideal model for high resolution analysis. …
Read PDFAortic dissection is a life-threatening cardiovascular emergency with limited pharmacological options. This study focuses on elucidating the multi-target and multi-pathway mechanisms through which morusin mitigates aortic dissection progression, integrating network pharmacology, single-cell transcriptomics and experimental …
Read PDFStructural lipidomics provides comprehensive information on lipidomes, and there is great interest in applying it to single-cell analysis for accurate cell phenotyping and lipid pathway studies. However, structural lipidomics relies on tandem mass spectrometry analysis …
Read PDFRecent advancements in single-cell multi-omics technologies have significantly improved our ability to explore cellular heterogeneity at an unprecedented resolution. These innovations enable the simultaneous profiling of genomic, transcriptomic, proteomic, and epigenetic data at the single-cell …
Read PDFHypertrophied adenoids in children can impair breathing and lead to obstructive sleep apnea (OSA), often accompanied by abnormal growth and weakened stamina and immunity. However, the cause of the pathological transformation in these originally immune-enhancing …
Read PDFThe rise of single-cell sequencing has revolutionized the exploration of drug resistance, revealing the crucial role of cellular heterogeneity in advancing precision medicine. By building computational models from existing single-cell drug response data, we can …
Read PDFCoronary artery disease (CAD) is a primarily inflammatory condition caused by atherosclerosis in the coronary arteries. Increasing evidence suggests that endoplasmic reticulum (ER) stress is closely involved in the development of CAD. In the arteries …
Read PDFTraditional bulk RNA sequencing often masks cell-to-cell variability, leading to a loss of individual heterogeneity information. Single-cell RNA sequencing (scRNA-seq) preserves cellular heterogeneity by reverse-transcribing, amplifying, as well as sequencing mRNA molecules from individual cells, …
Read PDFCell viability assays are essential tools in biomedical research and drug development. Artificial intelligence (AI) offers the potential to simplify these assays by predicting cell viability directly from brightfield microscopy images, but current models lack …
Read PDFEarly and accurate detection of diseases is of great significance for enhancing treatment outcomes and improving patient prognosis. The emergence of single-cell sequencing technology has brought new opportunities for this. This technology breaks through the …
Read PDFBACKGROUND: Intrauterine adhesion (IUA) is prevalent in women of childbearing age and can affect pregnancy outcomes or even lead to infertility. Currently, there is no effective clinical cure. METHODS: We profiled more than 100, 000 …
Read PDF<h4>Background</h4>Chronic obstructive pulmonary disease (COPD) and osteoporosis are common comorbid conditions, both of which impose a significant health burden. This research employs single-cell data and Mendelian randomization analysis to pinpoint genes associated with both conditions …
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