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Eukaryotic diversity is largely microbial, with macroscopic lineages (plants, animals, and fungi) nesting among a plethora of diverse protists. Our understanding of the evolutionary relationships among eukaryotes is rapidly advancing through 'omics analyses, but phylogenomic …
Read PDFThe evolutionary history of eukaryotic supergroups has been investigated primarily by large-scale phylogenomics, but one major hindrance to continued progress is that some major eukaryotic groups have extremely sparse sampling. The phylum Telonemia is one …
Read PDFThe 2025 Tutzing Workshop, held at the Evangelische Akademie on the shores of Lake Starnberg, continued a long tradition of highly integrative meetings focused on the biology and evolution of basal metazoans. The meeting was …
Read PDFDinoflagellates are an abundant and diverse group of protists that inhabit aquatic environments worldwide. They are characterized by numerous unique cellular and molecular traits, and have adapted to an unusually broad range of life strategies, …
Read PDFApicomplexans are a diverse phylum of single-celled protists that are all obligate animal symbionts. The most well-studied members are severe pathogens of humans and livestock, such as the causative agents of malaria, cryptosporidiosis, toxoplasmosis, and …
Read PDF<h4>Background</h4>Brain metastasis (BrM) is a leading cause of mortality in patients with lung adenocarcinoma (LUAD). Extracellular vesicles (EVs), which carry bioactive molecules, play a critical role in tumor microenvironment remodeling and exhibit metastatic organotropism, holding …
Read PDFField Asymmetric Ion Mobility Spectrometry (FAIMS) is an indispensable tool in single-cell and low-input proteomics. Here, we show that tuning FAIMS resolution via electrode temperature modulation improves sensitivity. We demonstrate that lowering FAIMS resolution broadens …
Read PDFBACKGROUND: Proteins represent the dynamic downstream products of genes, and have the potential to substantially increase understanding of the complexities of cancer biology. Undifferentiated pleomorphic sarcoma (UPS) is a rare disease, the proteomic landscape of …
Read PDF<h4>Background</h4>Multi-omics technologies have increasingly been applied to systemic autoimmune rheumatic diseases (SARDs), yet the global research landscape and thematic evolution of this field remain unclear.<h4>Objective</h4>This study intends to systematically review the application status and development …
Read PDFFLT3-mutated acute myeloid leukemia (AML) remains difficult to treat due to frequent resistance to FLT3 inhibitors like midostaurin. In this study, we observed a progenitor-like CD38<sup>+</sup>CD45RA<sup>+</sup> leukemic cell population that may be associated with midostaurin …
Read PDFdiaPASEF improves ion utilization and sensitivity by synchronizing quadrupole isolation with trapped ion mobility separation, making it suitable for single-cell proteomics. We present Full-DIA, a deep learning-driven software that enhances proteome coverage, quantitative accuracy, and …
Read PDFIn this perspective, we discuss the current challenges and opportunities in multi-omics, a rapidly evolving approach that integrates multiple molecular layers to advance our understanding of complex biological systems. As biomedical research moves toward precision …
Read PDFPituitary neuroendocrine tumors are frequently invasive, with cavernous sinus invasion leading to poor treatment outcomes and high recurrence. Regional differences within these tumors remain poorly understood, hindering targeted therapy development. Here, we present the first …
Read PDFOvarian tissue cryopreservation is a fertility preservation strategy available to prepubertal patients undergoing gonadotoxic treatment who are at risk of developing premature ovarian insufficiency or for those whose treatment cannot be delayed. While cryopreserved tissue …
Read PDFLung cancer is the leading cause of cancer-related deaths globally. Non-small cell lung cancer (NSCLC) accounts for approximately 85% of all lung cancer cases, and drug resistance severely undermines treatment efficacy. This review summarizes recent …
Read PDFAll eukaryotes share a single-celled ancestor from ∼1.5 to 1.8 billion years ago, the so-called last eukaryotic common ancestor (LECA). Roughly half of gene families found in modern eukaryotes were already present in LECA, forming …
Read PDFPrimary open-angle glaucoma (POAG) is a leading cause of irreversible blindness driven by elevated intraocular pressure from compromised aqueous outflow. While genome-wide association studies have identified numerous risk loci, specific candidate proteins and their cellular …
Read PDFTurquoise killifish (Nothobranchius furzeri) are naturally short-lived vertebrates that recapitulate key aspects of human aging. However, the molecular and cellular causes of systemic aging in killifish are poorly understood. Here we ask whether killifish undergo …
Read PDFHuman skeletal muscle is composed of highly heterogeneous single muscle fibers (multinucleated single cells) that are commonly classified as fast or slow fiber types, yet proteoform-resolved characterization of individual human muscle fibers remains lacking. Herein, …
Read PDFLysosomal storage, characterized by the progressive accumulation of undigested substrates in the lysosomal lumen, is a primary driver of various lysosome-related diseases. However, single-cell proteomic remodeling during lysosomal storage remains elusive, and the cellular responses …
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