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<h4>Introduction</h4>With their noninvasive capabilities for molecular-level analysis of biological tissues and cells, Raman spectroscopy and microscopy have become important tools in medicine and medical diagnostics.<h4>Methods</h4>In this work, we describe a reliable and repeatable technique for …
Read PDFComplex and dynamic networks of molecules are involved in human diseases. Single-cell and spatial multiomics approaches have created new avenues for understanding the pathogenesis and diagnosis of diseases. Cell connections and characteristics in diseases may …
Read PDFBreast cancer can develop over a wide age range and tumors in younger women differ from those in older women. Aging alters the spatial context of early tumors and may explain these differences, but breast …
Read PDFSex has a key role in disease susceptibility (in particular, autoimmunity). Sex differences in the immune system originate from genes and their interactions with both intrinsic and extrinsic factors. However, the cellular-level factors influencing sexual …
Read PDFGuillain-Barré syndrome (GBS) is an autoimmune disorder characterized by acute peripheral demyelination, leading to progressive muscle weakness and sensory loss. While CD4⁺ T cells and circulating macrophages are implicated in autoimmune neuropathies, the compositional dynamics …
Read PDFRapid advancements in technology enables the measurement of multimodal data at single-cell resolution, but with emerging modalities that are characterized by weak correlations with other modalities. Several computational approaches attempt to integrate these weakly linked …
Read PDF<h4>Background</h4>Chronic obstructive pulmonary disease (COPD) is characterized by persistent inflammation, structural remodeling, and irreversible airflow limitation, but the cellular mechanisms that sustain chronic inflammatory remodeling remain poorly understood.<h4>Methods</h4>We integrated newly generated single cell transcriptomic data …
Read PDFProtein phosphorylation regulates diverse cellular processes, yet its analysis at the single-cell level remains challenging due to the low abundance of phosphoproteins. Here, we present a highly sensitive system for profiling phosphorylation of kinase substrates …
Read PDFMultiplexing technologies label cells with molecular tags, allowing cells from different donors to be pooled together for sequencing. Although this approach enhances cell throughput, eliminates batch effects, and enables doublet detection, limitations of hashtag-based labelling …
Read PDFUnderstanding the organization and regulation of neurotransmission at the level of individual neurons and synapses requires tools that can track and manipulate transmitter-specific vesicles in vivo. Here, we present SynaptoTagMe, a suite of genetic tools …
Read PDFFoundation Models (FMs) have made significant strides in both industrial and scientific domains. In this paper, we evaluate the performance of FMs for single-cell sequencing data analysis through comprehensive experiments across eight downstream tasks pertinent …
Read PDF<h4>Introduction</h4>Understanding how cells respond to internal and external inputs requires investigating cells within three-dimensional (3D) environments, which better mimic physiological conditions. Compared to two-dimensional (2D) systems, 3D cultures more accurately simulate tissue architecture, including cell-cell …
Read PDFHuman buccal mucosa contains epithelial, stromal, and resident immune cell populations, but isolating these cells as a high-viability single-cell suspension is technically challenging. Here, we describe a gentle dissociation workflow for human buccal punch biopsies …
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Read PDFSingle-cell nanoencapsulation (SCNE) enables the direct integration of synthetic materials with living cells, forming cell-in-shell structures that augment native cellular functions without genetic modification. While SCNE has advanced applications in cytoprotection and cell-surface engineering, its …
Read PDFType 2 diabetes (T2D) and Alzheimer's disease (AD) are both increasing exponentially worldwide. T2D has also been identified as one of 14 modifiable risk factors for dementia, but the mechanism is unknown. T2D could promote …
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