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Therapeutic blockade of the PD-1/PD-L1 signaling pathway is the focus of tumor immunotherapy. However, drug resistance and irAEs induced by PD-1/PD-L1 inhibitors have emerged as major limitations affecting survival outcomes in patients with cancer. Macrophages …
Read PDFCell type annotation in single-cell RNA sequencing is a critical bottleneck, with existing automated methods facing limitations in accuracy, interpretability, and generalization to novel cell types. Although Large Language Models (LLMs) have recently shown potential …
Read PDFThe skin is the largest human organ and a site of substantial disease burden, yet its cellular and molecular organization across the body is largely undefined. Here we construct an organ-wide single-cell spatial atlas of …
Read PDFIntracellular crystallization is an emerging approach in structural biology that bypasses the need for protein purification. In 2024, the InCellCryst pipeline was introduced for structural studies of intracellular crystals by serial X-ray crystallography. Serial crystallography …
Read PDFThe generation of complex traits involves the coordinated interplay of multiple gene networks. Elucidating the function of transcriptional cis-regulatory elements (CREs) in regulating gene expression is crucial for understanding complex regulatory pathways and improving our …
Read PDFSingle-cell cloning (SCC) is a technique for the production of a pure clone from a single parental cell. SCC has become a fundamental process in biological research. It has many crucial applications, ranging from the …
Read PDFAccurate and prompt tumor diagnosis plays a critical role in surgical decision-making to minimize damage to patients with benign tumors and maximize the cure rate of cancer patients. Although imaging, molecular testing, and histopathology assist …
Read PDFMulticellular organisms develop from a single cell by repeated rounds of cell division, differentiation, and death, which can be represented as a single-cell phylogenetic tree. Genetic lineage tracing allows us to investigate this development by …
Read PDFAfrican swine fever virus (ASFV) infection leads to severe lung lesions in the host, yet detailed insights into the <i>in vivo</i> immune response of the lung microenvironment to ASFV infection at the single-cell level remain …
Read PDFTumor-associated macrophages (TAMs) constitute a critical immune cell population within the tumor microenvironment (TME) and exhibit high functional heterogeneity. In this study, a pan-cancer atlas comprising 28 TAM subtypes was systematically constructed by integrating single-cell …
Read PDFUnderstanding nanoparticle-cell interactions at the single-cell level is essential for designing next-generation nanomedicines. Here, we explored flow cytometry as a single-cell technique for label-free quantification of nanoparticle-cell interactions. We demonstrated the use of conventional flow …
Read PDFMicrobial optogenetic tools can regulate gene expression with spatial and temporal precision, offering excellent potential for single-cell resolution studies. However, bacterial optogenetic systems have primarily been deployed for population-level experiments. It is not always clear …
Read PDFSingle-cell chromatin accessibility analysis enables high-resolution dissection of regulatory elements and gene regulatory mechanisms. However, standardized and comprehensive analysis workflows remain limited. In this study, we present a streamlined pipeline for analyzing single-cell chromatin accessibility …
Read PDFHeat is a crucial factor in all biological processes; therefore, measuring heat change can be a powerful tool for monitoring bioprocesses and metabolism, analyzing biomolecular interactions, and studying cells. The insights gained from thermal measurements …
Read PDFThe unicellular red algae, Cyanidiophyceae, that diverged early during Archaeplastida (algal and plant) evolution, occupy a variety of extreme habitats that are inhospitable for most other eukaryotes. With the use of modern genomics and genetics …
Read PDFLipidomics provides detailed insight into lipid metabolism and cellular function, but conventional workflows typically rely on bulk samples that mask cellular heterogeneity. Advances in analytical chemistry are enabling lipid analysis from extremely limited material, driving …
Read PDFUnderstanding cell migration is essential not only for fundamental biology but also for the development of targeted disease therapies. Traditional <i>in vitro</i> cell migration assays typically rely on optical microscopy to capture cell movements and …
Read PDFAmpullary carcinoma (AC) is a rare epithelial cancer, representing around 0.2% of all gastrointestinal malignancies worldwide, with an estimated incidence of 0.73 per 100,000 people. The rarity of AC, combined with the intricate anatomy of …
Read PDFThe skin acts as the first barrier protecting the human body against the external environment. Transcriptional heterogeneity in human skin is widely confirmed, but the regulatory mechanisms remain largely unexplored. In this study, we employed …
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