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Advances in single-cell sequencing and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technologies have enabled detailed case-control comparisons and experimental perturbations at single-cell resolution. However, uncovering causal relationships in observational genomic data remains challenging due …
Read PDFBrain metastasis remains a significant cause of morbidity and mortality for cancer patients with limited treatment options. Single-cell RNA-seq has greatly expanded our ability to study cancer and metastasis, particularly in our assessment of tumor …
Read PDFSingle-cell genomics offers novel insights into genomic heterogeneity within cell populations, reframing our understanding of human development, tumorigenesis, and aging. However, constrained by the picogram-scale DNA templates of individual cells, Whole-Genome Amplification (WGA) remains a …
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Read PDFThe zebrafish lateral line system is a sensory network made up of neuromasts, which contain hair cells to detect water flow. Neuromasts signal via sensory afferent neurons and their activity is modulated by efferent neurons. …
Read PDFThe molecular programs governing early female gonadal development in livestock remain incompletely characterized. Here, we generated a single-cell transcriptomic atlas of bovine fetal ovaries spanning sex determination to early folliculogenesis and performed an integrated cross-species …
Read PDFSingle-cell RNA sequencing (scRNA-seq) data are inherently high-dimensional, and most analysis tools reduce this complexity by projecting the data into a low-dimensional latent space before performing downstream analyses. However, the resulting representations are often entangled, …
Read PDFImmune checkpoint blockade (ICB) induces durable responses in a subset of breast cancer patients, yet many show limited benefit from anti-PD-1/PD-L1 therapy. Understanding differences in the tumor microenvironment (TME) and tumor-intrinsic immune evasion between responders …
Read PDFUnderstanding how individual cells behave is essential for uncovering the complexity of biology and disease, yet most single-cell technologies provide only static molecular snapshots. We present the High-throughput Single-Cell Omni-functional Profiling Engine (HiSCOPE), a microfluidic …
Read PDFOBJECTIVE: High heterogeneity and poor therapeutic response are major challenges in hepatocellular carcinoma (HCC). This study aimed to identify core hepatocyte subsets and key genes driving HCC progression via a multi-omics approach to inform precise …
Read PDFThe evolution of distinct lung architectures in birds (tubular air capillaries) and mammals (alveoli) represents a classic example of convergent evolution, yet their cellular differences remain poorly characterized. We present the comparative single-cell atlas of …
Read PDF<h4>Background</h4>Single-cell multi-omics sequencing technologies enable profiling various cellular aspects, offering valuable biological insights. Integrating unpaired multi-omics data, which involves profiling different modalities from distinct cells within the same overall population, remains challenging yet crucial for …
Read PDFMass spectrometry-based single-cell metabolomics (SCM) reveals the inherent heterogeneity of individual cells among seemingly identical cell types. Fast-scanning and high-resolving mass analyzers provide the sensitivity and specificity required to probe minuscule amounts of biological material. …
Read PDFGlobal disability from osteoarthritis (OA) remains a staggering burden, yet disease-modifying pharmacological therapies remain limited. Current evidence supports OA as a whole-joint disease in which subchondral bone remodeling acts as an early and active driver …
Read PDFTargeted, small-molecule therapeutics have improved patient survival across cancer types, with >20 tyrosine kinase inhibitors (TKIs) receiving FDA approval as cancer therapies. While the initial TKI response is often promising, it is typically transient due …
Read PDFMultimodal profiling of circulating tumor cells (CTCs) and non-tumor circulating cells was performed in six patients with advanced colorectal cancer to identify biological programs beyond enumeration-based analyses. Across all patients, we identified 3,981 CTCs characterized …
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