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Cellular mechanotransduction, mediated by specialized structures such as microvilli, regulates processes ranging from tissue homeostasis to disease progression. Existing tools for microvilli-specific biomechanical intervention suffer from limited spatiotemporal precision and non-physiological constraints, restricting mechanistic studies …
Read PDFCell lineage relationship studies in developmental and regenerative biology have been greatly advanced using techniques such as fluorescent labeling driven by cell-type-specific promoters. Nevertheless, unbiased non-invasive tools for distinguishing cell lineages are inevitably desired. Mitochondrial …
Read PDFHigh-resolution, high-throughput single-cell omics has transformed our understanding of autoimmune disease pathogenesis. We synthesise recent single-cell omics advances across six autoimmune diseases-systemic sclerosis, systemic lupus erythematosus, Sjögren's syndrome, ankylosing spondylitis, IgG4-related disease and rheumatoid arthritis. …
Read PDFBulk organelle‑fractionation masks cell‑to‑cell heterogeneity, and existing microfluidic methods cannot reliably reconnect each isolated organelle to its parent cell, an essential capability for multiomics readouts. VacTrap, a high-throughput microfluidic device that isolates and spatially indexes …
Read PDFSurvival analysis is fundamental to cancer research. Advances in technology have enabled an increasing number of cohort-level cancer studies to incorporate single-cell sequencing alongside clinical survival data. However, no effective strategy currently exists for directly …
Read PDF<h4>Background</h4>Distant metastasis of osteosarcoma (OS) is one of the major factors contributing to poor patient prognosis, and its underlying mechanisms remain incompletely understood. In this study, we employed single-cell RNA sequencing (scRNA-seq) to assess intratumoral …
Read PDF<h4>Background</h4>Immune checkpoint blockade (ICB) has improved outcomes for a subset of colorectal cancer (CRC) patients, yet the cellular determinants that drive heterogeneous treatment responses remain insufficiently understood. The extent to which ICB reshapes immune populations …
Read PDFTime course single-cell RNA sequencing (scRNA-seq) enables researchers to probe expression dynamics at the resolution of individual cells. Analyzing this data poses several challenges, including deconvolving the contributions of time and cell type, discriminating true …
Read PDFTesticular development and spermatogenesis are critical for male reproduction, but their molecular mechanisms in Dezhou donkeys remain understudied. This study used single-cell RNA sequencing (scRNA-seq) to analyze testicular tissues from Dezhou donkeys at juvenile (2 …
Read PDFInactivated vaccines are critical to COVID-19 immunization, yet the cellular and molecular mechanisms driving pediatric responses warrant detailed characterization. We use single-cell transcriptomics to delineate the immune landscape of peripheral blood mononuclear cells in children …
Read PDFAdvances in single-cell sequencing have enabled AI-driven foundation models with powerful data representation. However, their practical use is limited by real-world data sparsity, heterogeneity, and poor interpretability. To overcome these, we introduce CellVQ. To enhance …
Read PDFPulmonary fibrosis is a prevalent, chronic, and fatal illness that poses considerable risks to life and health. <i>Actinobacillus pleuropneumoniae</i> (<i>A. pleuropneumoniae</i>) is an archetypal bacteria responsible for inducing significant pulmonary fibrosis, resulting in substantial economic …
Read PDF<h4>Background</h4>Axillary lymph node (LN) metastasis significantly impacts breast cancer (BC) prognosis. The role of the systemic immune environment in promoting metastasis remains unclear.<h4>Objectives</h4>This study describes peripheral blood immune alterations associated with LN metastasis in BC …
Read PDFMacrophages are pivotal innate immune cells that play essential roles in pathogen recognition, inflammation modulation, and tissue repair during pulmonary infections. Macrophages have remarkable plasticity that is shaped by diverse external stimuli to adapt to …
Read PDFInfluenza A virus (IAV) infection has a wide clinical spectrum, from mild illness to life-threatening pneumonia, yet the underlying immune determinants of disease remain poorly defined. Here, we generated a large-scale single-cell transcriptomic atlas from …
Read PDFMitochondrial DNA (mtDNA) mutations occur more frequently than nuclear mutations and are associated with various diseases. While single-cell sequencing enables mtDNA variant heteroplasmy analysis, a holistic view of mtDNA mutational landscapes in individual cells has …
Read PDFChromatin conformation capture technologies have revealed the complex 3D organization of the genome and its key regulatory role. Single-cell Hi-C (scHi-C) maps this architecture at single-cell level, but its sparse nature makes data interpretation challenging, …
Read PDF<h4>Objectives</h4>To characterise the immune cellular landscape and paired B-cell and T-cell receptor repertoires of hypertrophic adenoids in children with obstructive sleep apnoea.<h4>Methods</h4>We performed 10x Genomics 5' single-cell RNA sequencing with paired V(D)J profiling on adenoid …
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