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Acinar cells are the predominant cell type of the pancreas. However, in single-cell RNA sequencing datasets from healthy murine pancreas, acinar cells typically represent less than half of the total cells. Here, we developed a …
Read PDFCurrent single-cell profiling technologies enable the capture of multiple cellular modalities, providing valuable insights into complex biological systems. While a substantial amount of single-cell multimodal data has been generated and accumulated, most of these datasets …
Read PDFHead and neck squamous cell carcinoma (HNSCC) is highly heterogeneous, with variations driven by human papillomavirus (HPV) status and sex. However, existing single-cell RNA sequencing (scRNA-seq) studies are often limited in sample size and lack …
Read PDFClustered regularly interspaced short palindromic repeats (CRISPR) screens represent a transformative force in biological discovery, enabling the unbiased interrogation of gene function in a wide range of applications. Traditional screening approaches predominantly hinge on cell …
Read PDFLineage tracing using endogenous mitochondrial DNA (mtDNA) variants holds great promise for reconstructing the lineage histories of individual cells, with broad applications in oncology, developmental biology, and regenerative medicine. Unlike synthetic DNA barcoding techniques, mitochondrial …
Read PDFForensic deoxyribonucleic acid (DNA) interpretation is limited less by genotyping technology than by the biochemical and inferential effects of mixed, low-template, and environmentally complex traces. Single-cell and single-molecule strategies, including the United Kingdom Research and …
Read PDF<h4>Background</h4>Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline, β-amyloid (Aβ) plaque accumulation, neurofibrillary tangle formation, and chronic neuroinflammation. Increasing evidence suggests that ferroptosis, an iron-dependent form of regulated cell death driven …
Read PDFPrimary central nervous system lymphoma (PCNSL) is a rare, aggressive subtype of diffuse large B-cell lymphoma (DLBCL) with distinct biology. We present the first integrative single-cell transcriptomic atlas comparing PCNSL and systemic DLBCL (sDLBCL), profiling …
Read PDFDirect reprogramming (DR) involves inducing cell differentiation by converting somatic cells directly into target cells without bypassing induced pluripotent stem cells. Small molecule-induced DR reduces tumorigenic risk relative to transcription factor-triggered DR. However, identifying small …
Read PDFDown syndrome (DS), caused by trisomy of chromosome 21, is the leading genetic cause of intellectual disability, yet the mechanisms disrupting fetal brain development remain unclear. We performed single-cell transcriptomic and chromatin accessibility profiling of …
Read PDFThe distribution of biomolecules is essential information for understanding biological systems. Here, we present a protocol for 2D and 3D reconstruction of tissue images at the single-cell level by integrating microscopy and mass spectrometry imaging …
Read PDFMacrophages are pivotal in the progression of metabolic dysfunction-associated steatohepatitis (MASH), yet their specific markers remain elusive. Herein, we employed an integrated bioinformatics strategy, combining single-cell and bulk transcriptomic data from human MASH livers, to …
Read PDFThe retina is a dynamic neural tissue that lines the posterior of the eye cup and transfers visual inputs from the world to our brain for processing. However, exposure to stress, injury, and disease can …
Read PDFTranscriptional regulators reflect cellular heterogeneity and are key for prognostic modeling. Given the poor prognosis of stomach adenocarcinoma (STAD), regulator-derived signatures are vital for risk stratification. Using multi-stage scRNA-seq data, we delineated the transcriptional regulatory …
Read PDFMAPK and NF-κB pathways regulate macrophage responses to microbial stimuli. Repeated exposure to lipopolysaccharide (LPS) induces endotoxin tolerance, a state in which inflammatory cytokine production is suppressed while antimicrobial functions are preserved. Although regulatory mechanisms …
Read PDFCell migration and morphology are fundamental to various biological processes, including tissue development, wound healing, and cancer progression. In this study, we investigated the migration and morphological characteristics of HeLa cells cultured on different extracellular …
Read PDFMultiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system (CNS) characterized by multifocal inflammation and axonal degeneration, driven by innate and adaptive immune cells. The Janus Kinase (JAK)/Signal Transducers and Activators …
Read PDFAs primary sites for oxygen consumption and energy production via oxidative phosphorylation (OXPHOS), mitochondria play a central role in the regulation of bioenergetics and generation of key metabolic intermediates for myogenic cell growth. Common methods …
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