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Microvascular rarefaction is a predominant pathological hallmark of chronic kidney disease (CKD), functioning simultaneously as a catalyst and consequence of progressive renal compromise. Although endothelial senescence constitutes a cardinal mediator of microvascular attrition in CKD, …
Read PDFBACKGROUND: Single cell spatially resolved transcriptomics (ST) has revolutionized molecular profiling by providing the visualization of gene expression within its native tissue architecture, enabling insights into cellular heterogeneity, tumor microenvironment (TME) composition, and the molecular …
Read PDFCell-type-specific spatially variable genes (ctSVGs) integrate cell-type composition with spatially varying gene expression, revealing novel insights into molecular mechanisms through spatial transcriptomics. However, the emerging methods devoted to ctSVG detection have not been systematically evaluated, …
Read PDF<h4>Background</h4>Relapsed or refractory (R/R) B-cell non-Hodgkin's lymphoma (B-NHL) remains a major therapeutic challenge despite CD19-directed chimeric antigen receptor (CAR) T-cell therapies, with treatment failure often driven by antigen escape and limited CAR-T persistence. Dual targeting …
Read PDFRepeated implantation failure (RIF) presents a significant challenge to in vitro fertilization (IVF), and abnormalities in the endometrium often play a crucial role in RIF. To enhance and deepen our understanding of tissue context in …
Read PDFPeripartum cardiomyopathy (PPCM) is a rare form of acute heart failure that develops in women toward the end of pregnancy or early postpartum. No effective, specific treatment for PPCM is available and heart transplantation or …
Read PDF<h4>Background</h4>Per- and polyfluoroalkyl substances (PFAS), persistent environmental contaminants, are associated with increased Prostate cancer (PCa) risk. However, their molecular mechanisms are poorly defined.<h4>Methods</h4>We employed a comprehensive computational and experimental framework. The toxicological profiles of PFOA …
Read PDFBACKGROUND: Melanoma, a type of skin cancer that can spread to other parts of the body, currently lacks highly precise individualized treatment options. METHODS: We performed multi-omics integration on The Cancer Genome Atlas Skin Cutaneous …
Read PDFThe liver circadian clock coordinates hepatic lipid metabolism, bile acid synthesis, and glucose homeostasis through interlocking transcription-translation feedback loops. Disruption of this temporal organization is increasingly recognized as a shared pathological feature across the chronic …
Read PDFSkin cancer is one of the most common malignant tumors worldwide, with its mortality rate showing a significant upward trend, thereby increasing the disease burden on patients and society. With the rapid development of next-generation …
Read PDFFibrosis induced immune exclusion is a hepatocellular carcinoma (HCC) hallmark, underscoring the key role of cancer-associated fibroblasts (CAFs) in immune regulation. Through HCC spatial multi-omics data and integrating pan-cancer scRNA-seq profiles of CAFs under immune …
Read PDF<b>Background:</b> Radiotherapy resistance in breast cancer remains a major clinical challenge. The key molecular determinants and cellular populations driving this resistance are not fully understood. <b>Methods:</b> A radiotherapy resistance (RR) gene panel was identified from …
Read PDFDespite significant advances in understanding lung development, the intricate cellular interactions and spatial organization of the developing human lung remain incompletely defined. Spatial transcriptomics enables gene expression profiling within the native tissue context, providing unprecedented …
Read PDF<h4>Background</h4>Basophils are implicated in various diseases including allergies, but a comprehensive single-cell characterization of human basophils has yet to be performed. Here, we aimed to generate a single-cell omics-based reference resource of circulating human basophils, …
Read PDFMulti-kilobase knock-ins (KIs) are a necessary, yet challenging type of genome editing to create and characterize in cell lines and animals. The combination of rAAV donor transduction and electroporation of single-cell mouse embryos with Cas9/gRNA …
Read PDFHEK293 is a preferred cellular platform to produce viral vectors including adeno-associated viruses (AAV). However, HEK293 cells were shown to be genomically unstable and many HEK293 cell lines having distinct genotypes and phenotypes have been …
Read PDFLeukocytes at the maternal-fetal interface have critical functions during pregnancy in the prevention of maternal reactivity towards fetal alloantigens, suppression of excess inflammation and promotion of placental angiogenesis. Failed maternal immune adaptations to pregnancy can …
Read PDF<h4>Background</h4>Postmenopausal osteoporosis (PMO) develops as a result of pathological cross-tissue interactions. However, current experimental paradigms are constrained by their single-tissue focus, hindering efforts to discover systemwide regulatory genes.<h4>Objective</h4>We aimed to discover conserved genetic regulators of …
Read PDFUnderstanding changes in gene expression and cell-cell signaling among spatial regions in diseased tissues adds critical biological information to understanding mechanisms. Here, we present a protocol to investigate molecular transcriptional drivers within intact murine tissue …
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