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Thyroid cancer (TC), the most prevalent endocrine malignancy worldwide, encompasses a broad spectrum of biological behaviors ranging from indolent microcarcinomas to lethal anaplastic variants. Despite advancements in standard care, critical clinical "bottlenecks" persist, including the …
Read PDFThe ability to resolve rare and transient cellular states is critical for understanding metastasis, immune evasion, and therapy resistance in cancer, yet these dynamic processes often escape detection by conventional sequencing and imaging approaches. Recent …
Read PDFThe acceleration of climate change poses a growing threat to human health, particularly by exacerbating non-communicable diseases (NCDs) such as cardiovascular and respiratory conditions. Rising global temperatures amplify air pollution and environmental toxins, disproportionately affecting …
Read PDF<h4>Background</h4>Emerging evidence suggests a significant link between the gut microbiome and cardiovascular health, yet its role in the pathophysiology of heart failure has been underexplored. This systematic review and meta-analysis synthesize available research on how …
Read PDFAn exciting feature of nanopore sequencing is its ability to record multiomic information on the same sequenced DNA molecule. Well-trained models allow the detection of nucleotide-specific molecular signatures through changes in ionic current as DNA …
Read PDF<h4>Objectives</h4>CRISPR-Cas9 nucleases are widely used to introduce targeted DNA double-strand breaks (DSBs) for genome engineering, but the long-term impact of these lesions on local epigenetic information remains poorly characterized. In a companion research article, we …
Read PDF<h4>Background</h4>The characterisation of circulating nucleic acid biomarkers in liquid biopsy based diagnostics holds great potential to transform the landscape of early cancer detection and screening. These tests are increasingly incorporating information beyond the primary sequence, …
Read PDFWe developed a method for repeated and sequential retrieval of arbitrary DNA elements. A click chemistry process was used to conjugate the DNA molecules onto a plastic surface within the interior of microcentrifuge tubes. For …
Read PDF<h4>Background</h4>Native long-read DNA sequencing simultaneously captures genetic variants and epigenetic modifications from single molecules, but preserving molecular length and base modifications currently depends on cold-chain infrastructure that limits access to well-resourced settings.<h4>Results</h4>We demonstrate that ensilication, …
Read PDF<b>Background:</b> Long-read, single-CpG-resolution sequencing is redefining the information-to-depth ratio in epigenomics. While conventional methylome analysis often requires high coverage, we propose a scalable pipeline designed to extract high-density regulatory logic from shallow sequencing data. <b>Methods:</b> …
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Read PDFIndividual CpG methylation signatures characterize each mammalian cell type, thereby offering a powerful readout for clinical and diagnostic applications. Here, we apply this principle to in vitro cellular systems, using cell-free DNA released into the …
Read PDFGlatiramer acetate (GA) is a first-line disease-modifying therapy for multiple sclerosis (MS) with well-established moderate efficacy and high safety, yet its mechanisms of action remain incompletely understood. DNA methylation plays a significant role in MS …
Read PDFThe AT-rich interactive domain-containing protein 2 (ARID2) is a core component of the chromatin-remodeling complex that regulates transcription by modulating nucleosome positioning. Here we have characterized six ARID2 commercial antibodies for western blot, immunoprecipitation, and …
Read PDFHigh-grade serous ovarian carcinoma (HGSOC) is characterised by profound genomic instability and limited durable responses to standard therapy, leading to poor prognosis. The use of next-generation sequencing technologies has improved understanding of its molecular landscape, …
Read PDFLarge-scale single-cell genomics projects have revolutionized our understanding of human immune variation. Yet most studies to date have been Eurocentric, limited in cell-type resolution, or restricted to a single data modality. The newly published Chinese …
Read PDFAtherosclerotic cardiovascular disease (ASCVD) remains a leading cause of morbidity and mortality worldwide. Preventing ASCVD is of utmost importance; however, a large proportion of preventable cases is not discovered early enough to initiate relevant treatment. …
Read PDF<h4>Background/aim</h4>Previous studies suggest that thyroid cancers in the isthmus are associated with more aggressive behavior and unfavorable patient outcomes compared to lobar tumors. This study aimed to characterize the molecular features of isthmus thyroid cancer.<h4>Materials …
Read PDFDeep learning, as a pivotal branch of machine learning, has demonstrated remarkable potential in advancing crop science by effectively integrating genomics and phenomics. This review systematically outlines the application of diverse deep learning architectures-such as …
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