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A novel, strictly anaerobic, slightly alkaliphilic, halotolerant, peptide- and amino acid-utilizing bacterial strain, SD1<sup>T</sup>, was isolated from a hypersaline lake in Western Australia. The strain stained Gram-negative and was a motile, spore-forming rod. The strain …
Read PDFLong-standing, continuous blurring and controversies in the field of phylogenetic interspecies relations, associated with insufficient explanations for dynamics and variability of speeds of evolution in mammals, hint at a crucial missing link. It has been …
Read PDFColorectal cancer (CRC) is the third most commonly diagnosed cancer and the second leading cause of cancer-related death globally. Most CRCs arise from colorectal adenomas (CRAs), particularly advanced adenomas, which are recognized as critical precancerous …
Read PDFLysine methylation is a regulatory post-translational modification with diverse roles across both histone and non-histone proteins. Despite its biological relevance, comprehensive characterization of lysine methylation remains analytically challenging due to its low stoichiometry, subtle mass …
Read PDF<b>Background:</b> Asthma involves chronic inflammation linked to metabolic reprogramming, but how metabolites reshape epigenetics through posttranslational modifications remains unclear. <b>Methods:</b> We used house dust mite (HDM)-induced asthmatic mice with multiomics analyses (metabolomics, posttranslational modification-proteomics, and …
Read PDFReduction of disulfide bonds in proteins followed by selective modification of Cysteine (Cys) residues is a common practice in proteomics experiments to facilitate digestion into peptides and ultimately identification by mass spectrometry (MS). Due to …
Read PDFMass spectrometry (MS) is indispensable for high-throughput quantitation of protein expression. But protein function is regulated by factors beyond abundance alone. Here, we evaluate two supercharging reagents, dimethyl sulfoxide (DMSO) and m-nitrobenzyl alcohol (mNBA), in …
Read PDFThe Hevea brasiliensis is the only commercial source of natural rubber. In natural rubber production, exogenous ethylene is widely used as a stimulant for increasing rubber latex yield. To reveal the potential regulation mechanisms for …
Read PDFDupuytren's disease is a fibroproliferative disorder of the palmer fascia (PF) characterised by flexion contractures in the hand. Dupuytren's disease can be treated surgically, but disease recurrence rates are high, potentially due to continual production …
Read PDFAcute myeloid leukemia (AML) remains challenging to treat due to extensive genetic heterogeneity, high relapse rates, and treatment-related toxicity. Although drug combinations offer therapeutic promise, their selection is often empirical. Here, we introduce Combinatorial Proteome …
Read PDFSUMOylation is an understudied post-translational modification (PTM) linked to diverse physiological and pathological processes. Advances in enrichment strategies, combined with mass spectrometry (MS), have enabled large-scale mapping of SUMOylation sites. Here, we reanalyzed publicly available …
Read PDFProtein-l-isoaspartate <i>O</i>-methyltransferase (PIMT), encoded by <i>PCMT1</i>, is a repair enzyme that corrects isoaspartyl lesions, preserving protein structure and function. While indispensable for neuronal integrity, its role in red blood cells (RBCs) and transfusion outcomes is …
Read PDF<b>Background:</b> Mass spectrometry (MS)-based immunopeptidomics has emerged as the gold standard for profiling HLA-bound peptides, yet detection remains challenging due to their non-tryptic nature, variable lengths, and lack of basic residues, which limit ionisation and …
Read PDF<h4>Background</h4>Sjögren's syndrome (SS) is a chronic, systemic inflammatory disorder primarily characterized by dry eyes and dry mouth, often involving multiple organ systems. The disease's heterogeneous clinical presentation and the absence of non-invasive, specific biomarkers complicate …
Read PDFPost-translational modifications (PTMs) contribute greatly to the diversity of the human proteome by affecting protein structure, function, interactions, stability, localization, and more. The study of PTMs is essential to understand various cellular functions, disease mechanisms, …
Read PDFAbsence of the endosomal SNAREs vti1a and vti1b results in perinatal death and severe neuronal phenotypes in mice, while lack of one of these proteins results in minor phenotypes. Proteomic differences were investigated to obtain …
Read PDFThis dataset contains mass spectrometry-based proteomic profiles of human brain organoids cultured on Earth for 30 days, then maintained aboard the International Space Station (ISS) for an additional 30 days, with matched ground controls that …
Read PDFMethylglyoxal (MGO) is a reactive metabolic byproduct of glycolysis shown to accumulate in highly glycolytic cells such as cancer cells. MGO reacts with proteins to form covalent adducts in a process termed glycation, and MGO-glycation …
Read PDFExtracellular vesicles (EVs) have gained increasing attention with their intriguing biological functions and their molecular cargoes serving as potential biomarkers for various diseases, including cancers. A relatively lower abundance of EV proteins compared to cellular …
Read PDF<h4>Background</h4>Critical illness myopathy is a common and devastating consequence of critical care, causing dramatic loss of muscle mass and function in intensive care unit patients. Functional deficits often exceed the loss in muscle mass and …
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