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Deterioration of the blood-brain barrier (BBB), including impaired neurovascular uncoupling, contributes to cognitive decline in aging. The BBB is formed principally by brain microvascular endothelial cells (ECs), and ECs throughout the body are enriched for …
Read PDFTranscriptomic approaches such as cap analysis of gene expression (CAGE) enable the identification of transcription start sites (TSS) and the quantification of promoter activity. However, these techniques require large RNA input amounts and cannot profile …
Read PDFCannabis sativa has undergone over 10,000 years of domestication, resulting in extensive genetic and phenotypic diversity among cultivated chemotypes. Increased medical and recreational use of specialized metabolites accumulating in cannabis glandular trichomes-primarily the cannabinoids ∆9-tetrahydrocannabinol …
Read PDF<h4>Background</h4>Intestinal epithelial stem cells (SCs) and their transit-amplifying (TA) progeny are critical for mucosal repair and regeneration. However, their behaviour under chronic inflammatory conditions, such as those observed in Inflammatory Bowel Disease (IBD), remains incompletely …
Read PDFMüller glia arise from late-stage retinal progenitor cells (RPCs) as a distinct lineage that diverges from neurogenic trajectories. Here, we identify the maternally imprinted gene Plagl1 as a key transcriptional regulator of gliogenesis in the …
Read PDFC-type lectin receptors (CLRs) are a family of pattern recognition receptors that sense diverse microbial and damage-associated molecular patterns. Many CLRs, like Mincle and Dectin-3, are upregulated by TLR4 signaling. In Alcohol-associated Hepatitis (AH), where …
Read PDFPleiotrophin (PTN), a heparin-binding growth factor with potent mitogenic and angiogenic activity, has emerged as a key regulator of mammary gland biology and a potential driver of breast cancer progression. This review integrates current evidence …
Read PDF<h4>Objective and design</h4>To define the role of Ten-Eleven Translocation (TET) proteins in Crohn's disease (CD)-associated inflammation through integrative human and mechanistic studies.<h4>Material</h4>Publicly available CD transcriptomic and DNA methylation datasets, and primary mononuclear cells and ileal …
Read PDFHigh-grade serous ovarian cancer (HGSC) is a heterogeneous disease. RNA sequencing (RNAseq) of bulk solid tissue is of limited use in these populations due to heterogeneity. Single-cell RNA-seq (scRNA-seq) allows for the identification of diverse …
Read PDFHigh-frequency longitudinal RNA sequencing has emerged as a powerful approach for capturing dynamic transcriptional responses to therapeutic interventions, yet traditional differential expression analysis fails to identify genes with temporal variability that lack static expression differences. …
Read PDFThe mammalian circadian clock is an autoregulatory feedback process that is responsible for homeostasis in mouse livers. These circadian processes are well understood at the gene level; however, they are not well understood at the …
Read PDFThe transcription factor Sex-determining region Y-box protein 3 (SOX3) is well known for its critical roles in sex determination and cell differentiation; however, its function in antiviral innate immunity remains unexplored. This study uncovered how …
Read PDF<h4>Background</h4>Neuroblastoma, originating from the sympathetic neural crest, is the most prevalent extracranial solid tumor in children. Amplification of MYCN is a widely recognized indicator of poor prognosis in neuroblastoma. However, the structural properties of the …
Read PDFCombining allele-specific expression (ASE) analysis with single-cell RNA-seq can elucidate how genomic variation affects RNA expression at the single-cell level. We explore how experimental and computational choices impact the power of ASE-based methods and develop …
Read PDF<h4>Background</h4>Ad26.COV2.S is a recombinant, replication-incompetent human adenovirus serotype 26 (Ad26) vectored vaccine encoding a full-length SARS-CoV-2 spike protein in a prefusion-stabilised conformation. The genes and pathways which are differentially regulated following vaccination with Ad26.COV2.S, how …
Read PDFProtein quality control (PQC) systems are essential for cellular resilience to proteotoxic stress. Despite intensive study, functional redundancies in the system obscure the contributions of important individual genes. Here, we leverage transposon sequencing (Tn-seq) across …
Read PDFChemoreception through olfaction regulates essential fish behaviors, including reproduction. Fish olfactory receptor repertoire comprises four multigene families (olfactory receptors class C (OlfC), odorant receptor (OR), olfactory receptors class A (ORA), and trace amine-associated receptors (TAAR)), …
Read PDF<h4>Background</h4>Clear cell renal cell carcinoma (ccRCC) is the most common histological type of RCC and is marked by aggressive nature and poor survival. However, therapeutic options remain limited and yield suboptimal outcomes. Macrophages exhibit marked …
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