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Pregnant individuals are at increased risk of severe outcomes from COVID-19 infection, yet the immune responses elicited by vaccination during pregnancy remain poorly understood. In this study, longitudinal single-cell RNA sequencing (scRNA-seq) was performed on …
Read PDF<b>Background:</b> Pancreatic ductal adenocarcinoma (PDAC) has a profoundly immunosuppressive tumour microenvironment (TME) that limits anti-tumour immunity and contributes to resistance to immunotherapy. Although γδ T-cells can integrate innate and adaptive immune signals, their abundance, transcriptional …
Read PDFEfficient lipid mobilization from yolk is critical for avian embryonic development, yet the cellular mechanisms governing lipoprotein transport in yolk sac membrane endodermal epithelial cells remain poorly characterized. Single-cell RNA sequencing was performed on 9,037 …
Read PDF<h4>Background</h4>Lung adenocarcinoma (LUAD) is a predominant subtype of lung cancer associated with an unfavorable prognosis. However, the roles of the tumor microenvironment (TME) and Kynureninase (KYNU) in LUAD remain largely unclear. This study aimed to …
Read PDFPancreatic islets maintain glucose homeostasis through coordinated action of endocrine and affiliate cell types and are central to type 2 diabetes (T2D) genetics and pathophysiology. Our understanding of robust human islet cell type-specific alterations in …
Read PDFPhysiological and pathological processes are governed by networks of genes called gene regulatory networks (GRNs). By reconstructing GRNs, we can accurately model how cells behave in their natural state and predict how genetic changes will …
Read PDF<h4>Background</h4>Single-cell RNA sequencing (scRNA-seq) allows for the measurement of gene expression at the transcriptomic level with single-cell precision, thereby deepening our comprehension of cellular heterogeneity. However, the high dimensionality and sparsity of scRNA-seq data impede …
Read PDFOvarian cancer (OC) is a lethal gynecological malignancy with a low 5-year survival rate. The lack of preclinical models accurately capturing OC's early pathogenesis contributes to this poor prognosis. The domestic laying hen uniquely develops …
Read PDFTuberculosis-diabetes mellitus (TB-DM) is increasingly recognized as a syndemic in which chronic metabolic dysregulation amplifies tuberculosis severity, delays treatment response, and increases relapse and mortality. However, conventional systemic correlates soluble cytokines and bulk whole-blood transcriptomic …
Read PDF<h4>Background</h4>Acute-on-chronic liver failure (ACLF) is a life-threatening syndrome involving dysfunction of multiple immune cell types.<h4>Objective</h4>This study aimed to comprehensively depict the dynamic trajectory of immune responses throughout the disease course of HBV-related ACLF (HBV-ACLF).<h4>Design</h4>Single-cell RNA …
Read PDFThe rapid development of updated and new commercially available single-cell transcriptomics platforms provides users with a range of experimental options. Cost, sensitivity, throughput, flexibility, and ease of use all influence the selection of an optimal …
Read PDFCervical cancer is a prevalent malignancy among women, yet the involvement of neutrophils in its tumor microenvironment remains insufficiently explored. This study utilized single-cell RNA sequencing (scRNA-seq) to delineate neutrophil subsets and elucidate their roles …
Read PDFIschemic stroke (IS), a predominant cerebrovascular disorder contributing to global disability and mortality, characterizes by a complex, multi-tiered cascade of pathological processes. As the primary innate immune cells within the central nervous system (CNS), microglia …
Read PDF<h4>Background</h4>Papillary thyroid carcinoma (PTC) exhibits marked clinical heterogeneity that cannot be fully explained by tumor-intrinsic alterations alone. Emerging evidence suggests that immune cell senescence, particularly within B-cell compartments, may critically reshape the tumor immune microenvironment …
Read PDFSingle-cell RNA sequencing (scRNA-seq) is widely utilized in tumor research. However, platform-specific technical biases may impact data interpretation. This study compared the performance of droplet- and microwell-based scRNA-seq platforms in the analysis of clinical samples. …
Read PDF<h4>Background</h4>Psoriasis, an immune-mediated systemic inflammatory disease affecting skin, vessels, and joints, often co-occurs with depression. Routine depression screening is vital, as mood disorders link to inflammation, visible lesions, and functional limitations.<h4>Methods</h4>The study integrated Mendelian randomization …
Read PDFProper migration and differentiation of neuroblasts into neurons are essential for the development of a healthy nervous system. In this context, the asymmetrical migration of Caenorhabditis elegans Q neuroblasts provides a powerful model for studying …
Read PDF<h4>Background</h4>The urinary bladder contains a heterogeneous cell population. Multiple studies utilizing single-cell RNA sequencing techniques have uncovered the complex transcriptomic profile of cells in the healthy bladder.<h4>Objective</h4>The present study aims to map existing evidence on …
Read PDF<h4>Background</h4>Single-cell analysis has refined our understanding of cellular heterogeneity in glioma, yet RNA alternative splicing, a critical layer of transcriptome regulation, remains underexplored at single-cell resolution. Here, we present a pan-glioma single-cell alternative splicing analysis …
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