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<h4>Background</h4>Maternal obesity increases the risk of congenital anomalies and later-life metabolic disease in offspring. Still, underlying mechanisms remain unclear, particularly in extraembryonic lineages at the maternal-fetal interface, which remain poorly studied.<h4>Methods</h4>We jointly profiled gene expression …
Read PDFExosomes were isolated from cultures of fibroid explants and matched myometrial explants, and their RNA cargo was analyzed by next-generation sequencing to profile both long and short RNA species. Fibroid-derived exosomes (Fib-EXO) expressed the canonical …
Read PDFThe landscape of cellular abnormalities occurring during metabolic dysfunction-associated steatotic liver disease (MASLD) is not completely clarified. We investigated cell heterogeneity involved in progressive MASLD at single cell resolution in mice fed AMLN. Single cell …
Read PDFAccurate estimation of cell-type composition from bulk RNA sequencing (RNA-seq) is critical for dissecting the cellular basis of disease. Single-cell RNA sequencing (scRNA-seq) has emerged as a preferred reference for cell-type deconvolution. However, in practice, …
Read PDFBiliary atresia (BA), the leading cause of pediatric liver transplantation, is characterized by neonatal jaundice and progressive extrahepatic bile duct obstruction, yet its pathogenesis remains elusive. To uncover extrahepatic cholangiocyte injury, we generated patient-derived extrahepatic …
Read PDFMost genetic polymorphisms associated with complex traits are found in non-coding regions of the genome. Characterizing their effect presents a formidable challenge, and expression quantitative trait locus (eQTLs) mapping has been a key approach to …
Read PDFRecurrent point mutations in ribosomal proteins (RPs) RPL10 and RPS15 are found in T-cell acute lymphoblastic leukemia (T-ALL) and chronic lymphocytic leukemia (CLL), respectively. Furthermore, deletions of RPL5, RPL11, and RPL22 are frequent in hematologic …
Read PDFTranscriptional enhancers must locate target genes with precision. In mammals, topologically associating domains (TADs) guide this process, but the C. elegans genome lacks such organization despite containing over 30,000 putative enhancers. Using high-resolution Hi-C, we …
Read PDFRenal-resident macrophages (RMs) are essential regulators of kidney homeostasis and repair, yet the mechanisms governing RM niche regeneration after acute depletion remain poorly defined. To overcome these limitations, we have developed an inducible human CD59- …
Read PDFDermatophytes are important fungal skin pathogens affecting humans and animals worldwide. Although several virulence factors have been identified using genomic, proteomic, and transcriptomic approaches, their roles remain incompletely understood. In this study, we applied a …
Read PDFThe dysregulation of gene expression programs in the human atria during persistent atrial fibrillation (AF) is not completely understood. Here, we reanalyze bulk RNA-sequencing datasets from two studies (N = 242) and identified 755 differentially …
Read PDFThe fungal priority pathogen and basidiomycete, <i>Cryptococcus neoformans (Cn)</i>, causes lung and brain infection in predominantly immuno-compromised individuals and there is an urgent need for new treatment options. The pyrazolopyrimidine-based cyclin dependent kinase (CDK)7 inhibitor, …
Read PDFHaploinsufficiency of Dyrk1a, which encodes the dual-specificity tyrosine phosphorylation regulated kinase 1A (DYRK1A), has been causally linked to autism. Here we examined transcriptomic, electrophysiological and behavioral alterations in mice carrying a loss-of-function mutation of Dyrk1a …
Read PDFFlowering dogwood (<i>Cornus florida</i> L.) and Asian dogwood (<i>Cornus kousa</i> F. Buerger ex Hance) are popular deciduous ornamental trees native to a wide range of the eastern and southeastern United States and East Asia, respectively. …
Read PDF<b>Background/Objectives</b>: Non-coding RNAs provide new chances of targeting multiple oncogenic pathways. Many long non-coding RNAs (lncRNAs) are being characterized as relevant in cancer initiation, progression, and recurrence. <i>Mitochondrial D-loop 1 antisense lncRNA (MDL1AS)</i> is a …
Read PDF<h4>Introduction</h4>Partial gene duplications are structural variants that are challenging to interpret, particularly in the context of neurodevelopmental disorders. The ASH1L gene, associated with autism spectrum disorders and cognitive impairment, exemplifies the complexity of such variants. …
Read PDFAdvances in high-dimensional flow cytometry and single-cell RNA sequencing (scRNA-seq) have enhanced our understanding of the heterogeneity of tumor-infiltrating B cells (TIBs). Subpopulations of TIBs exhibit diverse, sometimes opposing roles in tumor control, influenced by …
Read PDFRibosome profiling is a powerful technique used to study gene expression on a transcriptome-wide scale. It involves sequencing of mRNA fragments protected by ribosomes from ribonuclease digestion. The initial steps commonly involve cell lysis followed …
Read PDF<h4>Background</h4>Neurodevelopmental disorders have a strong male bias that is poorly understood. The placenta provides molecular information about environmental interactions with genetics (including biological sex) that shape developmental processes in the brain. We investigate placental-brain transcriptional …
Read PDFPancreatic cancer (PC) is considered one of the deadliest cancers worldwide, and the number of PC-related deaths is expected to increase. Early diagnosis of PC is crucial for improving treatment outcomes. Despite improvements in overall …
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