Search the literature by keyword, topic, or author. Filter to articles with full text available.
2701 results
Human buccal mucosa contains epithelial, stromal, and resident immune cell populations, but isolating these cells as a high-viability single-cell suspension is technically challenging. Here, we describe a gentle dissociation workflow for human buccal punch biopsies …
Read PDFThe molecular mechanisms that produce cell structure are poorly understood. The giant ciliate <i>Stentor coeruleus</i> is a unicellular model organism whose large size, reproducible structure, and ability to heal wounds and regenerate allow the formation …
Read PDF<h4>Background</h4>Long noncoding RNAs (lncRNAs) participate in various critical regulatory steps during myogenesis. LncRNAs can encode small peptides, which can regulate gene expression through multiple mechanisms, thereby participating in key biological processes.<h4>Results</h4>The lncRNAs were screened between …
Read PDFAlterations to the community structure and function of the microbiome are associated with changes to host physiology, including immune responses. However, the contribution of microbe-derived RNAs carried by outer membrane vesicles (OMVs) to host immune …
Read PDFType 2 diabetes (T2D) and Alzheimer's disease (AD) are both increasing exponentially worldwide. T2D has also been identified as one of 14 modifiable risk factors for dementia, but the mechanism is unknown. T2D could promote …
Read PDFCandida albicans is the leading cause of vulvovaginal candidiasis (VVC), a multifactorial disease of the lower female reproductive tract. Despite numerous studies, the molecular-pathophysiology during VVC and its commensal state within the host remains elusive. …
Read PDF<h4>Background</h4>Malaria, caused by <i>Plasmodium</i> parasites, remains a major parasitic disease worldwide. Post-transcriptional gene regulation is essential for the intraerythrocytic development of <i>Plasmodium falciparum</i>, yet the functions of many parasite RNA-binding proteins remain poorly understood. Serine/arginine-rich …
Read PDFSomatic embryogenesis (SE), the process by which differentiated somatic cells are reprogrammed to form embryos, represents a unique manifestation of plant cell totipotency. Despite its fundamental and applied importance, the molecular mechanisms that initiate embryogenic …
Read PDFMatryoshka RNA virus 1 (MaRNAV-1) is a bi-segmented and single-stranded RNA virus associated with <i>Plasmodium vivax</i>, a cause of human malaria. Little has been uncovered about the epidemiology and ecology of this virus since its …
Read PDFProgranulin (PGRN) is a neurotrophic and anti-inflammatory factor produced mainly by neurons and microglia in the central nervous system. Progranulin haploinsufficiency causes frontotemporal dementia (FTD). It is unclear to what extent neuronal versus microglial PGRN …
Read PDFTumor-associated macrophages (TAMs) are key components of the colorectal cancer (CRC) microenvironment, yet the transcriptional programs associated with M2 states and their prognostic relevance remain incompletely defined. Here, single-cell RNA-seq analysis of CRC identified macrophages …
Read PDFIntronic polyadenylation (IPA) is a key mechanism driving transcriptome diversity, yet its detection and functional characterization remain challenging owing to complex splicing patterns and the complexity of intronic regions. Here, we introduce IPAseek, a dynamic …
Read PDF<h4>Background</h4>Metabolic dysfunction-associated steatohepatitis (MASH), the progressive phenotype of metabolic dysfunction-associated steatotic liver disease (MASLD), is well recognized for its increased risk of progressing to cirrhosis and hepatocellular carcinoma (HCC). However, early diagnosis and identification of …
Read PDFMyeloproliferative neoplasms (MPNs), particularly with myelofibrosis (MF), involve a disrupted perivascular hematopoietic niche, ultimately leading to bone marrow fibrosis. We asked if the transcriptome in cell-free RNA (cf-RNA) from the peripheral blood of patients with …
Read PDFVenous leg ulcers (VLUs) are chronic wounds in the lower limbs that cause significant morbidity. The underlying biology underpinning non-healing in VLUs is still poorly understood and differences in transcriptomic profiles may help elucidate biological …
Read PDFMicrobial volatile metabolites can enhance plant growth, yet the mechanisms by which plants perceive and transduce these signals are unknown. Growth of <i>Arabidopsis thaliana</i> Col-0 seedlings was found to be stimulated by volatiles from the …
Read PDFDetermining tumor-specificity of MHC-bound peptides is crucial for cancer immunotherapy development, yet current methods struggle with class II peptides and non-reference sequences. We introduce PepQueryMHC, an ultra-fast tool that integrates MHC-bound peptide sequences with translated …
Read PDFAlzheimer’s Disease represents the most significant form of neurodegenerative disease worldwide with progressive dementia and synaptic dysfunction. Though the accumulation of β-amyloid and hyperphosphorylated tau protein is the most observed pathological feature of AD, the …
Read PDFIron is essential for normal cellular function. Homeostatic responses to low iron availability have long been known to rely on posttranscriptional mechanisms. Poly C-binding proteins (PCBPs) are essential RNA-binding proteins that regulate alternative splicing (AS), …
Read PDFAcidosis is a hallmark of the tumor microenvironment and has been linked to aggressive cancer behavior, characterized by increased migration, invasion, and metastasis. We herein demonstrate that short-term exposure (24-72 h) to acidic extracellular pH …
Read PDF