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<h4>Background</h4>Glioma represents the most prevalent and lethal primary malignant tumor of the central nervous system, characterized by remarkable cellular heterogeneity and poor prognosis. Comprehensive characterization of glioma at single-cell resolution is essential for identifying novel …
Read PDFThe potential health hazards caused by microwave exposure have attracted increasing attention. Microwave radiation has been reported to induce oxidative stress in neural tissues, which is considered one of the primary mechanisms underlying its adverse …
Read PDFSepsis is a life-threatening systemic inflammatory syndrome often associated with immune dysregulation, multi-organ dysfunction, and high mortality. In recent years, single-cell transcriptomics has generated a wealth of data on immune cell heterogeneity, lineage reprogramming, and …
Read PDFRNA sequencing (scRNA-seq) has emerged as a transformative technology for dissecting cellular heterogeneity and immune complexity in livestock species. This review summarizes recent advances in the application of single-cell transcriptomics to livestock health, with a …
Read PDF<h4>Background</h4>The accurate prediction of cellular responses to perturbations, such as drug treatments, remains a pivotal challenge in single-cell transcriptomics. While numerous deep learning tools have been developed for this task, their systematic benchmarking across diverse …
Read PDFTumour heterogeneity is a key factor in cancer progression, with immune responses within the tumour microenvironment (TME) contributing significantly to treatment resistance and immunotherapy outcomes. Recent advances in single-cell RNA sequencing (scRNA-seq) have provided unprecedented …
Read PDFSingle-cell RNA sequencing (scRNA-seq) has become a cornerstone experimental technique in tissue biology, with gene expression data for over 100 million cells available in public repositories. The high dimensionality, sparsity, and technical noise inherent to …
Read PDFCell type annotation is a fundamental task in single-cell genomics. Although various methods have been developed for automatic annotation, they often function as black-box models lacking explainability, proper uncertainty estimation, and robustness for rare cell …
Read PDFPreeclampsia (PE) is a multisystem syndrome that manifests after 20 weeks of gestation, with a global incidence of 2%-8%, and is one of the leading causes of maternal and perinatal mortality. Its etiology is complex, …
Read PDFThe human nasal region arises from neural crest and placodal lineages, yet its early development remains poorly understood owing to limited fetal tissue access and structural complexity. Here we present an integrated single-nucleus and spatial …
Read PDFThyroid cancer exhibits pronounced cellular heterogeneity, yet the mechanisms underlying epithelial-mesenchymal transition (EMT) and tumor microenvironment (TME) remodeling remain incompletely understood. In this study, single-cell RNA sequencing of 92,849 cells from 20 thyroid tissue samples …
Read PDFChildren with Down syndrome have a 150-fold increased risk of developing myeloid leukaemia (ML-DS). Unusually for a childhood leukaemia, ML-DS arises from a preleukaemic state, termed transient abnormal myelopoiesis (TAM), via a conserved sequence of …
Read PDF<h4>Background</h4>Atherosclerosis (AS) progression is profoundly influenced by dynamic T-cell heterogeneity and functional plasticity.<h4>Main body</h4>This review consolidates the latest insights derived from single-cell transcriptomic technologies, which are transforming our understanding of the role of the immune …
Read PDFThe tumor microenvironment (TME) has emerged as a pivotal determinant of tumor progression, immune evasion, and therapeutic response, making it a central focus in modern oncology. Single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to …
Read PDFThis study aimed to elucidate the molecular mechanisms and regulatory pathways driving the onset and progression of periodontitis (PD). Four transcriptomic datasets from the Gene Expression Omnibus database were integrated to identify differentially expressed genes …
Read PDFGuillain-Barré syndrome (GBS) is an autoimmune disorder characterized by acute peripheral demyelination, leading to progressive muscle weakness and sensory loss. While CD4⁺ T cells and circulating macrophages are implicated in autoimmune neuropathies, the compositional dynamics …
Read PDF<h4>Background</h4>Chronic obstructive pulmonary disease (COPD) is characterized by persistent inflammation, structural remodeling, and irreversible airflow limitation, but the cellular mechanisms that sustain chronic inflammatory remodeling remain poorly understood.<h4>Methods</h4>We integrated newly generated single cell transcriptomic data …
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