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<h4>Background</h4>Distant metastasis of osteosarcoma (OS) is one of the major factors contributing to poor patient prognosis, and its underlying mechanisms remain incompletely understood. In this study, we employed single-cell RNA sequencing (scRNA-seq) to assess intratumoral …
Read PDFThe brain displays the richest repertoire of post-transcriptional mechanisms regulating mRNA translation<sup>1-11</sup>. Among these, alternative splicing has been shown to drive cell-type specificity and, when disrupted, is strongly linked to neurological disorders<sup>12-17</sup>. However, genome-wide measurements …
Read PDFThis protocol describes sc‑rDSeq, a scalable, droplet‑based method for full‑length, strand‑specific total RNA sequencing at single‑cell resolution. The protocol uses a refined set of 220 ribosomal‑depleted sequences (rDS) primers that selectively exclude ribosomal RNA during …
Read PDF<h4>Background</h4>Immune checkpoint blockade (ICB) has improved outcomes for a subset of colorectal cancer (CRC) patients, yet the cellular determinants that drive heterogeneous treatment responses remain insufficiently understood. The extent to which ICB reshapes immune populations …
Read PDF<h4>Background</h4>The ovary is a central female reproductive organ responsible for producing oocytes and secreting steroid hormones. To investigate the molecular similarities and potential evolutionary origins of the ovary across vertebrates, we integrated publicly available single-cell …
Read PDFTesticular development and spermatogenesis are critical for male reproduction, but their molecular mechanisms in Dezhou donkeys remain understudied. This study used single-cell RNA sequencing (scRNA-seq) to analyze testicular tissues from Dezhou donkeys at juvenile (2 …
Read PDFInactivated vaccines are critical to COVID-19 immunization, yet the cellular and molecular mechanisms driving pediatric responses warrant detailed characterization. We use single-cell transcriptomics to delineate the immune landscape of peripheral blood mononuclear cells in children …
Read PDFGlioma, characterized by its highly invasive nature, presents significant challenges in prognosis and treatment resistance. The advent of single-cell RNA sequencing (scRNA-seq) has facilitated a more nuanced understanding of the cellular and molecular landscapes of …
Read PDF<h4>Background</h4>Tuberculous pericarditis (TP) can progress to tuberculous constrictive pericarditis (TB-CP), a life-threatening fibrotic syndrome. However, the cellular architecture and intercellular circuits that link granulomatous inflammation to pericardial fibrosis remain poorly defined.<h4>Methods</h4>We performed single-cell RNA sequencing …
Read PDFPulmonary fibrosis is a prevalent, chronic, and fatal illness that poses considerable risks to life and health. <i>Actinobacillus pleuropneumoniae</i> (<i>A. pleuropneumoniae</i>) is an archetypal bacteria responsible for inducing significant pulmonary fibrosis, resulting in substantial economic …
Read PDFInfluenza A virus (IAV) infection has a wide clinical spectrum, from mild illness to life-threatening pneumonia, yet the underlying immune determinants of disease remain poorly defined. Here, we generated a large-scale single-cell transcriptomic atlas from …
Read PDF<h4>Background</h4>Differential diagnosis of pleural effusion remains challenging despite medical thoracoscopy (MT). We investigated whether integrating proteomic biomarkers with single-cell transcriptomics and genomic mutation profiling could improve diagnostic accuracy and reveal mechanistic insights.<h4>Methods</h4>We prospectively enrolled 564 …
Read PDF<h4>Background</h4>A link exists between decorin (DCN) and endometriosis, nevertheless, the role of DCN in this condition remains unclear. This study aims to bioinformatically characterize DCN expression, diagnostic value, and related signaling in endometriosis using single-cell …
Read PDFImmune checkpoint inhibitors (ICIs) have revolutionized cancer therapy, but their increasing clinical use has led to an increasing incidence of immune-related adverse events (irAEs), among which colitis and cardiotoxicity are particularly severe, compromising both quality …
Read PDF<h4>Background</h4>Diabetic cardiomyopathy (DCM) is a diabetes-related myocardial disorder causing fibrosis, hypertrophy, and progressive diastolic and systolic dysfunction. This study aims to explore how metabolic, inflammatory, and fibrotic mechanisms in non-cardiomyocytes drive DCM to reveal new …
Read PDFSingle-cell RNA-sequencing (scRNA-seq) studies over the past several years have provided unprecedented resolution into the transcriptomic landscape of both major and minor salivary glands. This technology enables the identification of diverse and functionally specialized cell …
Read PDFTo understand the heterogeneity of single-B cell responses to Metabolic Dysfunction-Associated Steatohepatitis (MASH), we performed Single-cell RNA sequencing (scRNA-seq) on single-B cells isolated from control and MCD-fed mice livers. Subsequent analyses included clustering, identification of …
Read PDF<h4>Background</h4>Bladder cancer (BLCA) exhibits a poor prognosis, highlighting the urgent need for reliable prognostic genes. Although succinylation is linked to tumor progression, its role in BLCA remains understudied. This study aimed to identify and validate …
Read PDFAberrant cell-cell communication (CCC) is a recognized hallmark of aging, yet comprehensive analyses of immune CCC-particularly in the context of healthy aging-remain limited. Using single-cell transcriptomics of PBMCs from centenarians (CEN), their offspring (CO), and …
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