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Single-cell RNA sequencing enables comprehensive analysis of cellular diversity across biological systems. While current batch correction methods can jointly define cell types across multiple conditions, individuals, or modalities, they typically require matching features or paired …
Read PDFOvarian cancer(OC) poses a significant clinical challenge due to its frequent peritoneal dissemination and development of chemotherapy resistance, contributing to poor patient outcomes. Chronic inflammation is a pivotal driver of disease initiation and progression. While …
Read PDFThe interactions between lytic phages and their hosts are typically studied in bulk culture, which obscures cell-cell differences in infection susceptibility or expression of protective factors. Here, we use bacterial single-cell RNA sequencing to profile …
Read PDF<h4>Background</h4>Neutrophils, which are polymorphonuclear leukocytes of the phagocytic system, serve as critical mediators of inflammation-induced injury and are implicated in various diseases. However, the role of neutrophils in primary Sjögren's syndrome (pSS) remains underexplored.<h4>Objectives</h4>Single-cell RNA …
Read PDFSingle-cell RNA sequencing data rely heavily on dimensionality reduction methods to uncover underlying structures and patterns. Nonlinear dimensionality-reduction methods such as uniform manifold approximation and t-distributed stochastic neighbor embedding have been routinely used for this …
Read PDFEndometriosis is a chronic hormone-responsive disorder linked to infertility, usually characterized by the presence of ectopic endometrium in the pelvis that disrupts local homeostasis. Advances in single-cell "omic" methods have revealed the remarkable cellular diversity …
Read PDFPeriodontitis (PD) and type 2 diabetes mellitus (DM) are bidirectionally associated through shared chronic inflammatory mechanisms. Although monocytes serve as central immune mediators in both pathologies, their functional heterogeneity and dynamic alterations in diabetic periodontitis …
Read PDF<h4>Background</h4>Sarcoidosis is a complex, multi-system granulomatous disease characterized by immune dysregulation and chronic inflammation, primarily affecting the lungs. To identify cell specific molecular changes associated with sarcoidosis development and progression, we aimed to characterize cellular …
Read PDFTranscriptome-wide association studies (TWAS) have successfully identified genes associated with complex traits and diseases, but most have been performed using bulk gene expression data, which aggregate signals across heterogeneous cell types. Population-scale single-cell RNA sequencing …
Read PDFPeripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) presenting with disseminated cutaneous involvement represents an aggressive disease subset with poor response to conventional chemotherapy and dismal prognosis. The tissue-specific molecular mechanisms driving cutaneous aggression remain poorly …
Read PDFSingle-cell multiomic sequencing technologies have offered unprecedented insights into cellular heterogeneity by jointly profiling gene expression and epigenetic landscapes at single-cell resolution. However, the application of these technologies remains limited owing to technical challenges and …
Read PDFThe domestic chicken (Gallus gallus) is a critical model for immunology and a major agricultural species, yet a comprehensive single-cell census of its immune architecture is lacking. Here, we constructed a high-resolution single-cell transcriptome atlas …
Read PDFTranslating high-resolution multiomics data into clinically actionable biomarkers is critical for overcoming therapeutic resistance and tumor heterogeneity in prostate adenocarcinoma (PRAD). To decode the complex immunosuppressive tumor microenvironment (TME) and identify robust prognostic targets, we …
Read PDFSingle-cell RNA sequencing (scRNA-seq) enables high-resolution characterization of cellular heterogeneity, but its rich, complementary structure across cells and genes remains underexploited, especially in the presence of technical noise and sparsity. Effectively leveraging this multi-scale structure …
Read PDF<h4>Background</h4>Migraine (MI), Ménière's disease (MD), and vestibular migraine (VM) share significant clinical and pathological similarities, particularly their link to neurological dysfunction and immune cell activity, though mechanisms remain poorly understood.<h4>Methods</h4>We utilized a single-cell RNA sequencing …
Read PDFWe utilized single-cell RNA sequencing (scRNA-seq) to investigate cellular heterogeneity and signaling networks in aortic dissection (AD) tissues compared to adjacent normal tissues. The analysis identified five smooth muscle cell (SMC) subtypes, with SMC2 linked …
Read PDFMicrovessels within atherosclerotic plaques are crucially involved in disease progression. Here, we generated a transcriptomic atlas of human atherosclerosis at single-cell resolution, encompassing 17,367 vascular endothelial cells (VECs) from five scRNA-seq studies, and verified key …
Read PDFSunlight is a fundamental immune-modulating factor with implications for the pathogenesis of autoimmune diseases such as multiple sclerosis (MS). MS incidence and severity increase with latitude, historically attributed to diminished ultraviolet (UV) exposure and lower …
Read PDF<h4>Background</h4>Hepatocellular carcinoma (HCC) is one of the most common types of cancer globally. However, HCC features poor prognosis due to complex pathogenesis and limitations of therapeutic approaches.<h4>Method</h4>To improve the prognosis of HCC patients, we analyzed …
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