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<h4>Background</h4>Hepatocellular carcinoma (HCC) represents a major global health burden, characterized by complex metabolic reprogramming and immunological dysregulation. This study aimed to elucidate the molecular mechanisms underlying HCC progression using integrative multi-omics analyses, with a specific …
Read PDFClustered regularly interspaced short palindromic repeats (CRISPR) screens represent a transformative force in biological discovery, enabling the unbiased interrogation of gene function in a wide range of applications. Traditional screening approaches predominantly hinge on cell …
Read PDFAortic aneurysm (AA) is a life-threatening vascular disorder lacking effective medical therapies. Gut microbiota contributes importantly to vascular homeostasis; yet, the underlying gene-microbiome interactions in AA remain unclear. Using two-sample Mendelian randomization, we identified and …
Read PDFCraniofacial development is a complex process that involves the specification of diverse and transient cell types. However, our understanding of these processes and the cell-types present during human craniofacial developmental remains limited. We address this …
Read PDF<h4>Background</h4>Focal cortical dysplasia (FCD) is a common neurodevelopmental disorder characterised by cortical malformations and is a major cause of drug-resistant epilepsy. FCD type I (FCDI) presents with architectural abnormalities of the neocortex but without cytological …
Read PDFHost-microbe dynamics during SARS-CoV-2 Omicron variant infection and recovery remain poorly understood, particularly regarding intracellular microbial communities in immune cells. We performed single-cell RNA sequencing of 191,417 peripheral blood mononuclear cells (PBMCs) from 57 individuals …
Read PDF<h4>Background</h4>Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline, β-amyloid (Aβ) plaque accumulation, neurofibrillary tangle formation, and chronic neuroinflammation. Increasing evidence suggests that ferroptosis, an iron-dependent form of regulated cell death driven …
Read PDFDirect reprogramming (DR) involves inducing cell differentiation by converting somatic cells directly into target cells without bypassing induced pluripotent stem cells. Small molecule-induced DR reduces tumorigenic risk relative to transcription factor-triggered DR. However, identifying small …
Read PDFPotato (Solanum tuberosum L.) is a globally important food crop with considerable nutritional and economic value. Heat stress significantly inhibits potato plant growth and tuber development, constraining the sustainable development of the potato industry. Currently, …
Read PDFDown syndrome (DS), caused by trisomy of chromosome 21, is the leading genetic cause of intellectual disability, yet the mechanisms disrupting fetal brain development remain unclear. We performed single-cell transcriptomic and chromatin accessibility profiling of …
Read PDF<b>Background:</b> Although emerging evidence indicates that major depressive disorder (MDD) raises the risk of developing ovarian cancer (OC) and worsens survival, the biological mechanisms underlying this relationship remain unclear. This study explores the MDD-OC association …
Read PDF<h4>Background</h4>Wilms tumor presents a heterogeneous tumor microenvironment. This study aimed to characterize the tumor microenvironment and identify prognostic genes to improve therapeutic strategies.<h4>Methods</h4>We integrated single-cell RNA sequencing and transcriptomic data from paired tumor and normal …
Read PDFMacrophages are pivotal in the progression of metabolic dysfunction-associated steatohepatitis (MASH), yet their specific markers remain elusive. Herein, we employed an integrated bioinformatics strategy, combining single-cell and bulk transcriptomic data from human MASH livers, to …
Read PDFMitoxyperilysis is a mitochondria-dependent membrane lysis process driven by innate immune and metabolic cues, yet its clinical relevance in melanoma remains unclear. We analyzed single-cell RNA-seq data (GSE215120) to quantify a mitoxyperilysis-related score (MRS), resolve …
Read PDFWeight-loss treatment is crucial for individuals with obesity to prevent various complications. The role of Immune cells in obesity has been recently recognized, whereas its translation into therapy requires identifying key target genes. We performed …
Read PDFBACKGROUND: Ovarian aging drives declining fertility, premature menopause, and systemic age-related diseases. Multiple different types of regulated cell death (RCD) pathways have been identified and play integral roles in diverse physiological and pathological processes. However, …
Read PDF<h4>Background</h4>The metabolic switch between oxidative phosphorylation (OXPHOS) and aerobic glycolysis is a fundamental feature of tumor biology. Its dynamic regulation during pancreatic neuroendocrine tumor (pNET) cell differentiation remains poorly characterized, especially at single-cell resolution.<h4>Methods</h4>We analyzed …
Read PDFProstate cancer (PCa) is frequently accompanied by benign prostatic hyperplasia (BPH), highlighting the need to reassess their correlation in tumor risk, malignant progression, and immune status to improve the early diagnosis and treatment of PCa. …
Read PDFMultiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system (CNS) characterized by multifocal inflammation and axonal degeneration, driven by innate and adaptive immune cells. The Janus Kinase (JAK)/Signal Transducers and Activators …
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