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BACKGROUND: Tumor evolution is driven by substantial cellular heterogeneity, yet its reconstruction remains challenging with conventional bulk sequencing approaches. Single-cell transcriptomic data provide opportunities to study clonal diversity and evolutionary dynamics, but accurate inference of …
Read PDF<h4>Background</h4>The breast cancer tumour microenvironment (TME) exhibits marked cellular and metabolic heterogeneity that contributes to disease progression and therapeutic resistance. Lactylation, a lactate-derived post-translational modification, has emerged as a potential link between metabolic reprogramming and …
Read PDFLegumes are important food crops and play a central role in sustainable agriculture through their ability to form symbiosis with rhizobia, soil bacteria that fix atmospheric nitrogen. Recent advances in single-cell and spatial transcriptomics, along …
Read PDFProstate cancer (PCa) remains a major global health concern, with a subset of patients progressing to aggressive disease despite advances in diagnosis and treatment. Epithelial-mesenchymal transition (EMT) plays a pivotal role in tumor invasion, metastasis, …
Read PDF<h4>Motivation</h4>Single-cell omics analysis has unveiled the heterogeneity of various cell types within tumors. However, no methodology currently reveals how this heterogeneity influences cancer patient survival at single-cell resolution. Here, we introduce scSurv, combining a Cox …
Read PDF<h4>Objectives</h4>Oligoarticular JIA (oligo JIA) is the most common JIA subtype and is often complicated by uveitis, a potentially sight-threatening comorbidity. Despite its prevalence, the immunopathogenic mechanisms underlying oligo JIA and its extra-articular manifestations remain poorly …
Read PDF<i>In vitro</i> kidney models play a crucial role in the study of renal development, (patho)physiology, drug development, and potential applications in bioartificial kidneys. Human <i>in vitro</i> kidney models have recently advanced significantly with the development …
Read PDFThe thymus is vital for T cell development, yet avian thymocyte maturation remains poorly characterized. Here, we present a single-cell transcriptomic atlas of thymic development in Zhedong White geese across embryonic, gosling, juvenile, and adult …
Read PDFRecent advancements in single-cell genomic and transcriptomic sequencing, in situ sequencing, and molecular imaging-based technologies have facilitated the examination of heterogeneity within microbial communities at the single-cell level. These cutting-edge methodologies permit the capture of …
Read PDFLymphatic malformations (LMs) are debilitating and potentially life-threatening diseases. However, the immune phenotype of circulating cells and underlying molecular mechanisms in LMs remain poorly understood. Here, we performed integrated single-cell RNA, T-cell receptor, and B-cell …
Read PDF<h4>Background</h4>Repair and remodeling following myocardial infarction (MI) are complex processes with a wide array of cellular and molecular mechanisms; however, the cell source mediating repair is still poorly understood in terms of heterogeneity and temporal …
Read PDFUnderstanding how to effectively treat neurological deficits after stroke remains difficult, as both the therapeutic targets and clinical outcomes are still uncertain. Although intermittent Theta burst stimulation (iTBS) has recently been shown to protect neural …
Read PDF<h4>Background</h4>Single-cell transcriptomics is a powerful approach to resolve cellular heterogeneity, yet its application in plants is constrained by challenges in tissue preparation, nuclei isolation, and transcriptome quality. Optimized experimental and computational workflows are essential to …
Read PDF<h4>Background</h4>Cutaneous melanoma is a highly aggressive malignancy characterized by significant heterogeneity, rapid progression, and variable treatment responses. Understanding the functional diversity of melanoma cells and their interactions with the tumor microenvironment (TME) is crucial for …
Read PDF<h4>Background</h4>The emergence of single-cell RNA sequencing (scRNA-seq) technology has revolutionized our capacity to study cell functions in complex tissue microenvironments. Traditional transcriptomic approaches, such as microarrays and bulk RNA sequencing, lacked the resolution to distinguish …
Read PDFDepot-specific fat deposition influences carcass yield and meat quality in ducks. To explore regulatory mechanisms, we profiled adipogenic progenitor cells (APCs) from pectoral muscle (PM), subcutaneous (SAT), and visceral adipose tissue (VAT) using single-cell RNA …
Read PDFSynchronicity is observed in many biological systems, including development, as seen in vertebrate body axis formation, fly eye development, and development of the social amoeba D. discoideum. Despite its prevalence, quantitative analyses of synchronicity at …
Read PDF<h4>Background</h4>Gestational diabetes mellitus (GDM) is a common pregnancy complication associated with hyperglycaemia, chronic inflammation and adverse health outcomes. Regulatory T cells (Tregs) are thought to contribute to GDM due to their role in suppressing inflammation. …
Read PDFHead and neck squamous cell carcinoma (HNSCC) is highly heterogeneous, with variations driven by human papillomavirus (HPV) status and sex. However, existing single-cell RNA sequencing (scRNA-seq) studies are often limited in sample size and lack …
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