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<h4>Introduction</h4>Plant development is shaped by environmental conditions, and its adaptation to climate change is crucial for biodiversity conservation. The extreme climate of the Qinghai-Tibet Plateau makes it an ideal system for studying plant adaptive strategies. …
Read PDFWhile single-cell RNA sequencing has advanced our understanding of cell fate, identifying molecular hallmarks of potency-a cell's ability to differentiate into other cell types-remains a challenge. Here we introduce CytoTRACE 2, an interpretable deep learning …
Read PDFThe progression of lung adenocarcinoma (LUAD) is influenced by polyamine metabolism, which modulates antitumor immunity, although the underlying mechanisms remain unclear. The present study investigates the role of polyamine metabolism-related genes (PMRGs) in LUAD using …
Read PDF<h4>Background</h4>The high dimensionality of data in single cell transcriptomics (scRNAseq) requires investigators to choose subsets of genes ("feature selection") for downstream analysis (e.g., unsupervised cell clustering). The evaluation of different approaches to feature selection is …
Read PDF<h4>Background</h4>Prostate cancer (PCa) is a leading cause of cancer-related death in men worldwide. The tumor microenvironment (TME), particularly its immune and stromal components, has a critical influence on PCa progression and patient outcomes. Identifying novel …
Read PDFThe tonsil is a highly compartmentalized organ in which different microanatomical structures orchestrate designated (immune) functions. We use this already well-studied tissue to survey imaging-based spatial transcriptomics data for studying immune responses in native tissue …
Read PDFInsect wing development involves tissue patterning, cell fate transitions, and hormone signaling, yet its spatiotemporal logic remains unclear. The silkworm, with large wing discs and defined stages, provides an ideal model for high resolution analysis. …
Read PDF<h4>Introduction</h4>Antlers are specialized bony appendages in mammals that exhibit the remarkable ability to periodically regenerate from the pedicles and then rapidly elongate to form bone. Previous studies have demonstrated that this intricate and precise biological …
Read PDFAortic dissection is a life-threatening cardiovascular emergency with limited pharmacological options. This study focuses on elucidating the multi-target and multi-pathway mechanisms through which morusin mitigates aortic dissection progression, integrating network pharmacology, single-cell transcriptomics and experimental …
Read PDFThis study addressed the significant challenges in understanding the specific immune landscape and predicting patient outcomes of COVID-19, particularly in severe cases. To support clinical practice, we aimed to build a comprehensive single-cell atlas of …
Read PDFHalo nevus is an autoimmune skin disorder characterized by autoreactive CD8<sup>+</sup> T cells that target and destroy melanocytes, resulting in a depigmented perilesional halo. Yet the upstream triggers of this activation remain unclear. Here, we …
Read PDFTesticular sex cord stromal tumor (TSCST) is a rare testicular tumor with unknown molecular mechanisms, tumor microenvironment (TME) and limited therapeutic options. To define the molecular mechanism and potential targets of TSCST, we combined single-cell …
Read PDFRecent advancements in single-cell multi-omics technologies have significantly improved our ability to explore cellular heterogeneity at an unprecedented resolution. These innovations enable the simultaneous profiling of genomic, transcriptomic, proteomic, and epigenetic data at the single-cell …
Read PDFWe develop the spatial dissimilarity method to uncover complex bivariate relationships in single-cell and spatial transcriptomics data, addressing challenges such as alternative splicing and allele-specific gene expression. Applying this method to detect alternative splicing in …
Read PDFCoronary artery disease (CAD) is a primarily inflammatory condition caused by atherosclerosis in the coronary arteries. Increasing evidence suggests that endoplasmic reticulum (ER) stress is closely involved in the development of CAD. In the arteries …
Read PDF<h4>Background</h4>Activating anaplastic lymphoma kinase (<i>ALK</i>) mutations define a clinically relevant subset of neuroblastoma (NB), yet <i>ALK</i>-linked transcriptional programs with robust prognostic value remain insufficiently characterized. This study aimed to identify <i>ALK</i>-associated transcriptional features across single-cell …
Read PDFTraditional bulk RNA sequencing often masks cell-to-cell variability, leading to a loss of individual heterogeneity information. Single-cell RNA sequencing (scRNA-seq) preserves cellular heterogeneity by reverse-transcribing, amplifying, as well as sequencing mRNA molecules from individual cells, …
Read PDFEarly and accurate detection of diseases is of great significance for enhancing treatment outcomes and improving patient prognosis. The emergence of single-cell sequencing technology has brought new opportunities for this. This technology breaks through the …
Read PDFBACKGROUND: Intrauterine adhesion (IUA) is prevalent in women of childbearing age and can affect pregnancy outcomes or even lead to infertility. Currently, there is no effective clinical cure. METHODS: We profiled more than 100, 000 …
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