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<h4>Background</h4>Obstructive sleep apnea-hypopnea syndrome (OSAHS) is a prevalent respiratory disorder characterized by intermittent hypoxia (IH), which promotes pulmonary complications. However, the cellular and molecular mechanisms underlying IH-induced lung remodeling remain poorly understood.<h4>Methods</h4>We performed comprehensive single-cell …
Read PDFSingle-cell RNA sequencing (scRNA-Seq) enables advanced analysis of cellular heterogeneity; however, due to data sparsity and noise, analyzing single genes can be challenging. While pathway activity scoring offers a robust alternative to standard single-gene analysis, …
Read PDFThe tumor microenvironment (TME) has a profound influence on the progression of lung adenocarcinoma (LUAD) and its response to therapy. We identified a tumor-promoting communication network based on single-cell RNA sequencing. This shows an SPP1 …
Read PDFRegulatory T cells (Treg) play immunosuppressive roles in lung cancer and are key biomarkers in cancer immunology. Using scRNA-seq and stereo-seq, we aimed to identify a novel circulating Treg subtype in non-small cell lung cancer …
Read PDFAccurate deconvolution of bulk and spatial transcriptomes is essential for studying tissue architecture and disease, yet remains challenged by unmodeled differences in cellular RNA content and cross-source heterogeneity. We introduce CSsingle, a unified deconvolution framework …
Read PDFTuberculous meningitis (TBM) is the most severe form of tuberculosis, with a fatality rate of 20% to 50% in treated individuals. Although corticosteroid therapy can increase survival in HIV-negative people with TBM, better antimicrobial and …
Read PDF<i>Salmonella</i> is a major foodborne pathogen that causes approximately 1.35 million infections annually in the US and remains a leading cause of poultry-associated foodborne illness. To improve chickens' resistance to this pathogen, it is important …
Read PDFWe previously demonstrated that blocking TGF-β with galunisertib, a safe, orally available small drug, reactivated latent SIV in vivo by shifting T cells toward a transitional effector phenotype. Here, we investigated the mechanisms underlying this …
Read PDF<h4>Introduction</h4>Kawasaki disease (KD) is an acute autoimmune vasculitis that predominantly affects children under 5 years of age. Although immune dysregulation is considered central to KD pathogenesis, the cellular heterogeneity and regulatory mechanisms underlying this process …
Read PDFPURPOSE: The complex tumor microenvironment (TME) in ovarian cancer (OC), particularly the role of macrophage clusters, plays a pivotal role in driving cancer progression and determining patient prognosis. Metabolic reprogramming is a hallmark of tumorigenesis …
Read PDFAging gradually impairs immune system function, yet its systemic features across immune organs remain poorly characterized in primates. Here, we perform single-cell transcriptomic profiling of bone marrow, spleen, mesenteric lymph nodes, and peripheral blood mononuclear …
Read PDF<h4>Background</h4>The human endometrium is a regenerative tissue relying on stem/progenitor cells. Endometrial mesenchymal stem cells (eMSCs) are typically enriched using perivascular markers like CD140b and CD146. However, the identity of more primitive and quiescent eMSC …
Read PDFProteins that impact phenotype and disease are often approximated by RNA expression, which poorly infers protein abundance. We developed DeepGxP, a deep-learning model trained on The Cancer Genome Atlas pan-cancer data, to predict protein abundance …
Read PDF<b>Background/Objectives</b>: Sex differences influence skin physiology, immune regulation, and disease susceptibility, but the cellular organization of sex-biased transcriptional programs in healthy human skin remains incompletely defined. We aimed to define sex-associated differences in cellular composition …
Read PDFNeural stem cells (NSCs) are self-renewing, multipotent cells capable of differentiating into neurons, astrocytes, and oligodendrocytes. NSCs reside in specific brain niches: the ventricular zone (VZ), the subventricular zone (SVZ) and the subgranular zone of …
Read PDFPeriodontitis is a chronic inflammatory disease affecting tooth-supporting tissues. Beyond microbial dysbiosis, host metabolic regulation plays a critical role. This study identified ornithine aminotransferase (OAT), a mitochondrial enzyme in amino acid metabolism, as associated with …
Read PDFIn recurrent spontaneous abortion (RSA), impaired decidualization due to decidual stromal cell (DS) apoptosis or pyroptosis disrupts pregnancy maintenance. RNA-binding proteins (RBPs) are crucial regulators of gene expression in reproductive disorders. However, the comprehensive profile …
Read PDF<h4>Background</h4>Acupuncture has been proposed as a therapeutic intervention for stroke recovery, yet the underlying molecular mechanisms remain poorly understood.<h4>Method</h4>In this study, we used a mouse model of hemorrhagic stroke induced by autologous blood injection to …
Read PDFBACKGROUND: Gastric cancer (GC) characterized by profound cellular heterogeneity, with the diversity and interactions of cells within the tumor microenvironment (TME) playing critical roles in tumorigenesis, metastasis, and therapeutic response. However, the underlying mechanisms remain …
Read PDF<h4>Background</h4>Advancements in single-cell RNA sequencing have enabled the analysis of millions of cells, but integrating such data across samples and methods while mitigating batch effects remains challenging. Deep learning approaches address this by learning biologically …
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