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Transcriptomics enables comprehensive, multiplexed characterization of cellular states, yet prevailing methods typically require cell fixation or lysis, precluding longitudinal analysis of RNA expression in living cells. Here, we present non-destructive transcriptomics by vesicular export (NTVE), …
Read PDFDifferential expression is a key application of imaging spatial transcriptomics, moving analysis beyond cell type localization to examining cell state responses to microenvironments. However, spatial data poses new challenges to differential expression: segmentation errors cause …
Read PDFTumors are composed of cellular populations with distinct genotypes and phenotypes, which dynamically evolve over time and during treatment. This process is known as clonal evolution, and it is difficult to reveal fine-scale clonal structure …
Read PDF<h4>Background</h4>Identifying novel prognostic indicators for patients with hepatocellular carcinoma (HCC) is critical to improving patient outcomes. Mapping the spatial composition of HCC tumors remains challenging. Therefore, this study aimed to identify prognostic genes and assess …
Read PDFAndrogenetic alopecia (AGA) manifests as progressive hair follicle (HF) miniaturization; however, its drivers remain poorly elucidated. Combining spatial and single-cell transcriptomics, we generate a concise single-cell atlas of anagen HFs in male AGA, revealing early …
Read PDFCrustaceans, such as shrimp and crabs, are pivotal to global aquaculture, yet their productivity is severely impacted by low-temperature stress. This study employs comparative transcriptomic and genomic analyses to elucidate the molecular mechanisms underlying crustacean …
Read PDF<h4>Background</h4>Oral squamous cell carcinoma (OSCC) exhibits heterogeneous therapeutic outcomes owing to various tumor microenvironments (TMEs). Previous studies have classified OSCC according to its molecular characteristics; however, clinical practice is limited by technical complexity.<h4>Methods</h4>A total of …
Read PDFPorcine deltacoronavirus (PDCoV) is an emerging enteropathogenic coronavirus that causes severe diarrhea in swine industries worldwide. However, the interactions between PDCoV and host cells remain poorly understood. In this study, we employed transcriptomic and proteomic …
Read PDFPorcine epidemic diarrhea (PED) is a severe viral disease caused by the porcine epidemic diarrhea virus (PEDV), leading to huge economic losses in the swine industry. Identifying therapeutic targets has long been a critical challenge …
Read PDFThe autoantibody reactome refers to the multidimensional repertoire of antibody reactivities against self-antigens across the human proteome or selected antigenic compartments. This offers a scalable systemic layer for precision immunology across spontaneous autoimmunity and treatment-induced …
Read PDFMetastatic breast cancer (MBC) remains the primary cause of mortality in breast cancer patients, driven by tumor heterogeneity, cellular evolution, and therapy-resistant clones. Traditional bulk transcriptomics, although informative, fail to capture rare subpopulations and context-specific …
Read PDFMetastasis remains the leading cause of cancer mortality, yet the factors that determine metastatic competence and the timing of its acquisition are not well understood. Using human breast cancer cohorts and multi-omics in preclinical models, …
Read PDFLichen planus (LP) is a chronic inflammatory disease of the skin and mucous membranes, marked by T cell infiltration and keratinocyte apoptosis. However, its immune microenvironment remains poorly understood. Using single-cell RNA sequencing, spatial transcriptomics, …
Read PDF<h4>Background</h4>Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related death worldwide and shows significant heterogeneity. Cadherin 13 (CDH13) is abnormally expressed in various malignancies, but its role in HCC and the tumor microenvironment remains …
Read PDF<h4>Purpose</h4>Dry eye disease (DED) is a multifactorial chronic disorder of the ocular surface, characterized by reduced tear secretion, tear film instability, and inflammation. Oxidative stress is pivotal in DED pathogenesis. Qingxuan Runmu Yin (QRY) has …
Read PDFHigh‑throughput transcriptomic technologies offer a systems‑level approach to unravel the mechanisms of complex immune disorders. Inflammatory Bowel Disease (IBD), a classic example of such disorders, involves intricate interactions between genetics, microbiota and immune dysfunction. The …
Read PDF<h4>Introduction</h4>Chronic alcohol consumption exacerbates the progression of periodontitis, the sixth most prevalent global disease affecting over 740 million people, though its underlying mechanisms remain poorly understood.<h4>Objective</h4>The study aims to elucidate the impact and mechanisms of …
Read PDFCellular morphology is tightly linked to function, but how subcellular transcript localization contributes remains unclear. Using microglia, the brain's resident macrophages, as a model, we combined multiplexed error-robust fluorescence in situ hybridization with immunohistochemistry to …
Read PDFSpatial transcriptomics enables the detection of rare or transitional tumor states not captured by bulk transcriptomics or immunohistochemistry (IHC). However, translating these spatially defined states into clinically relevant biomarkers is challenging, because signals from minor …
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