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<h4>Purpose</h4>Osteoporosis is a metabolic bone disorder characterized by reduced bone mass, impaired microarchitecture, and diminished bone strength, resulting in a significantly elevated risk of fractures. It is especially prevalent among older adults, particularly postmenopausal females, …
Read PDFThe endocrine system is crucial for maintaining overall homeostasis. However, its cellular signatures have not been elucidated during aging. Here, we conducted the first-ever single-cell transcriptomic profiles from eight endocrine organs in young and aged …
Read PDFWe introduce SCITO-seq2, an enhanced successor to SCITO-seq that integrates probe-based RNA detection with the established ultra-high-throughput protein profiling. SCITO-seq2 achieves robust quantification of transcripts and surface proteins across more than 100,000 cells, with a …
Read PDFCancer can recur when a subset of tumor cells, termed persister cells, survive therapy and re-enter the cell cycle. Through single-nucleus multi-omic profiling (single-nucleus RNA sequencing [snRNA-seq] and single-nucleus assay for transposase-accessible chromatin by sequencing …
Read PDFMicrobial communities function as dynamic societies where intercellular communication governs collective behaviors. However, mapping these interaction networks has remained a fundamental challenge in microbiology. This study aims to decode the social networks of complex bacterial …
Read PDFHigh-throughput single-cell RNA sequencing (scRNA-seq) has substantially advanced plant transcriptional landscapes. However, decoding cell-type-specific transcriptional regulation in non-model crops like tomato (<i>Solanum lycopersicum</i>) remains challenging. An integrated computational pipeline was applied using high-dimensional weighted gene …
Read PDFGlobal climate changes cause severe floods that hinder plant development and breeding. The phenomenon in which a single plant can significantly alter its leaf shape structure and physiology based on surrounding environments is known as …
Read PDFChronic kidney disease (CKD) is a leading and rapidly rising contributor to global mortality, yet actionable molecular targets remain limited. Here, we integrated human kidney single-cell RNA sequencing (scRNA-seq) with genetic association and causal inference …
Read PDF<h4>Background</h4>Paraneoplastic pemphigus (PNP) is a highly fatal autoimmune blistering disease that commonly occurs in patients with underlying benign or malignant neoplasms. It poses significant challenges for diagnosis and treatment. To date, the cellular and molecular …
Read PDFAlternative splicing (AS) plays a critical role in generating cellular transcriptomic heterogeneity. While single-cell RNA sequencing (scRNA-seq) has become a standard approach for exploring this heterogeneity, it remains challenging to accurately characterize splicing changes at …
Read PDFTissue makeup depends on the local cellular microenvironment. Spatial single-cell genomics enables scalable and unbiased interrogation of these interactions. Here we introduce Nicheformer, a transformer-based foundation model trained on both human and mouse dissociated single-cell …
Read PDFDepression frequently cooccurs with epilepsy (EP) and has become a focus of clinical management, but effective pharmacological interventions remain limited. In this study, single-cell RNA sequencing (scRNA-seq) data were analyzed to identify changes in oligodendrocyte …
Read PDF<h4>Background</h4>Hepatocellular carcinoma (HCC) is an aggressive cancer with limited therapies. Although transposable element-derived genes are increasingly linked to tumorigenesis, the role of the TIGD family in HCC remains unclear. This study examined the expression, clinical …
Read PDF<h4>Background</h4>Monocyte-derived macrophages (MoMFs) play key roles in liver inflammation and fibrogenesis, with their heterogeneity affecting metabolic dysfunction-associated steatotic liver disease (MASLD) progression. However, current therapeutic strategies targeting macrophage-mediated inflammation have shown limited clinical efficacy in …
Read PDFThe high mortality rate of metastatic cutaneous melanoma (SKCM) remains a major challenge in clinical treatment. This study used single-cell RNA sequencing (scRNA-Seq) technology to compare the differences between metastatic and primary tumour cells. By …
Read PDFAging is a regulated process marked by the accumulation of senescent cells, which remain viable but no longer divide. Senescent cells contribute to age-associated phenotypes and diseases, including osteoarthritis, dementia, and cancer, but their scarcity …
Read PDFGastrointestinal cancer is one of the most burdensome health threats worldwide, accounting for approximately one-quarter of all cancer incidences. Gastric and colorectal cancers are among the most prevalent and lethal malignancies of the gastrointestinal tract …
Read PDFImmune checkpoint inhibitors (ICIs) have transformed the advanced melanoma treatment landscape; however, a subset of patients achieve durable responses. Current biomarkers, such as PD-L1 expression and tumor mutational burden, offer limited predictive power due to …
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