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<b>Background</b>: Tumor invasion is the central barrier to effective immunotherapy in lung adenocarcinoma (LUAD) and glioblastoma. Cell adhesion signaling critically shapes tumor-microenvironment interactions, yet the upstream regulators coordinating these invasive programs at single-cell resolution remain …
Read PDFUnderstanding the development and differentiation of cardiac progenitor cells during the initial stages of embryogenesis is central to a complete understanding of vertebrate heart development. In zebrafish, cardiac specification begins during gastrulation; however, the single-cell …
Read PDFIncreasing prevalence of Alzheimer's disease, driven by population aging, highlights the need to investigate its underlying molecular mechanisms. Within this context, long non-coding RNAs (lncRNAs) have emerged as a key regulatory layer. To advance the …
Read PDFDendritic cells (DCs) are essential for the initiation and regulation of antitumor immune responses. Although DC vaccines hold great promise in personalized cancer immunotherapy, their efficacy remains limited by tumor-induced immunosuppression and insufficient DC maturation …
Read PDFSingle-cell spatial transcriptomes have demonstrated molecular and cellular diversity in the brain, but gene regulatory mechanisms underlying transcriptomic profiles and disease pathogenesis remain largely unknown in primates. Here we performed single-nucleus Assay for Transposase-Accessible Chromatin …
Read PDFSingle-cell RNA sequencing (scRNA-seq) has revolutionized our understanding of cellular heterogeneity in complex biological systems. However, analyzing and integrating scRNA-seq data poses unique computational challenges due to sparsity, high variability, and technical batch effects. Here, …
Read PDFSubtalar arthrodesis (SA) is a widely used salvage procedure for posttraumatic subtalar arthritis (PSA), but its underlying healing mechanisms remain poorly understood. The immune system plays a critical role in the union process after arthrodesis; …
Read PDFThe development of skeletal muscle is a biological process of great complexity and high regulation whereby there is a spatiotemporal coordination of interactions amongst various cell and molecular events. Recent advances in scRNA-seq and ST …
Read PDFGene regulatory networks (GRNs) are essential models for understanding gene expression, cell differentiation, and cellular function. Existing GRN resources primarily focus on transcriptomic data and often overlook the epigenetic mechanisms of gene regulation, limiting evaluation …
Read PDFEndometriosis (EMS) is a common gynecological disease that seriously affects women's health and quality of life. However, the detailed dynamic cellular and molecular mechanisms underlying EMS pathogenesis remain largely unknown. This study establishes a novel …
Read PDFChondrosarcoma (CHS) is a malignant bone tumor resistant to adjuvant treatment, with high-grade patients suffering an unfavorable prognosis. Therefore, a deeper insight into the tumor microenvironment is essential for developing effective treatments for advanced CHS. …
Read PDF<h4>Background</h4>Lung adenocarcinoma (LUAD) is a leading cause of cancer-related mortality worldwide. The tumor microenvironment (TME) plays a pivotal role in LUAD progression, but the specific molecular mechanisms driving malignancy and immune evasion remain incompletely understood. …
Read PDFTo enhance the understanding of cellular heterogeneity within the pediatric thyroid, single-nuclei RNA sequencing was used to recover 38,069 non-pathogenic cells from thyroid tissue of three female pediatric/young adult patients. The recovered cells were analyzed …
Read PDFSingle-cell transcriptomics enables precise characterization of cellular heterogeneity, but current pre-trained models relying solely on expression data fail to capture gene associations. We present scKGBERT, a knowledge-enhanced foundation model integrating 41 M single-cell RNA-seq profiles …
Read PDF<h4>Background</h4>The immune microenvironment of the three most common gynaecological malignancies-tubo-ovarian cancer, endometrial cancer and cervical cancer-has not been systematically studied, limiting clinical application.<h4>Methods</h4>This study analyses 272 389 CD45+ immune cells by integrating publicly available single-cell …
Read PDF<h4>Background</h4>Hepatocellular carcinoma (HCC) carries a dismal prognosis, yet the contribution of deubiquitination-an essential posttranslational regulator-to its progression remains poorly defined.<h4>Methods</h4>Single-cell RNA-seq profiles of 13 treatment-naïve HCC tumors were integrated with 374 TCGA and 243 ICGC …
Read PDF<h4>Aim</h4>Acute coronary syndrome (ACS) and ischemic stroke are major life-threatening conditions caused by atherosclerosis. Although the mechanisms of atherosclerosis appear to be broadly similar across different vascular beds, growing evidence suggests that there are morphological …
Read PDFThe immunosuppressive tumor microenvironment (TME) and intrinsic heterogeneity of ovarian cancer (OC) are primary drivers of therapeutic resistance and mortality. To deconvolute these complex dynamics and identify robust therapeutic targets, this study employed an integrative …
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