Search the literature by keyword, topic, or author. Filter to articles with full text available.
2701 results
Fluconazole is key for pulmonary cryptococcosis (PC), but its hepatotoxicity (HT) risk is unclear. This study characterized fluconazole-induced liver injury in HIV-negative PC patients and explored its mechanism. We integrated clinical cohort analysis (<i>n</i> = …
Read PDF<h4>Background</h4>Colorectal cancer (CRC) is the third most common malignancy worldwide, and the role of E2F transcription factor 6 (E2F6) in CRC remains controversial.<h4>Methods</h4>We analyzed E2F6 mRNA expression across 19 platforms (2,449 CRC patients and 1,328 …
Read PDFIntratumor macrophage infiltration plays a crucial role in various aspects of tumor immunity in colorectal cancer (CRC). However, the phenotypic heterogeneity, spatial distribution, and cellular interactions of these macrophages remain elusive. To overcome the limitations, …
Read PDFMultimodal spatial omics enables systematic characterization of tissue organization by jointly profiling transcriptomic, proteomic, metabolomic, and other spatially resolved modalities within their spatial context. A central challenge in realizing this potential is achieving robust spatial …
Read PDF<b>Background</b>: Precision oncology has traditionally relied on genomic biomarkers to guide therapy selection; however, static molecular profiling often fails to predict real-world responses to cytotoxic chemotherapy. Increasing evidence suggests that treatment outcomes are determined by …
Read PDFBreast and gynecologic cancers consist of two groups of complex solid tumors, each with unique genomic features, immune microenvironments, and treatment responses. Recent advances in next-generation sequencing, spatial profiling, and digital pathology have transformed diagnostic …
Read PDFDistal cholangiocarcinoma (dCCA) arises from the distal bile duct and is anatomically embedded within the pancreatic head, adjacent to abundant autonomic nerve plexuses. This unique location renders dCCA particularly prone to perineural invasion (PNI), a …
Read PDFBACKGROUND: This study investigates the role of fatty acid metabolism (FAM)-related genes in lymph node metastasis (LNM) and prognosis of lung adenocarcinoma (LUAD) and elucidates the underlying mechanisms. METHODS: Transcriptomic and single-cell RNA-seq data from …
Read PDF<h4>Background</h4>Panax ginseng faces continuous cropping obstacles due to autotoxicity from protopanaxadiol-type ginsenosides. Despite studies on individual ginsenosides being reported, the integrated impact of these compounds across different growth stages requires further investigation.<h4>Methods</h4>We employed multi-omics approaches …
Read PDF<h4>Background</h4>The development of spatial transcriptomics (ST) has enabled biologists to measure transcriptome data on an entire tissue and retain spatial information. It gives us the opportunity to fully understand the tissue microenvironment and identify spatial …
Read PDFBACKGROUND: Glioblastoma represents the most aggressive primary brain malignancy, characterized by a profoundly immunosuppressive microenvironment that limits therapeutic efficacy and drives disease progression. Myeloid cells—comprising tumor-associated macrophages, myeloid-derived suppressor cells, and dendritic cells—serve as central …
Read PDF<h4>Background and aims</h4>A comprehensive understanding of the liver niche during development provides insights into a unique, naturally occurring multifunctional multicellular environment. We applied spatial transcriptomic and histologic approaches to identify and histologically validate RNA-based markers …
Read PDFThe advancement of single-cell RNA sequencing and spatial transcriptomics has enabled the inference of cellular interactions in a tissue microenvironment. Despite advances in cell-cell interaction inference, methods capable of mapping the influence of ligands on …
Read PDFPulmonary arterial hypertension (PAH) is a severe, currently incurable lung disease characterized by endothelial injury and excessive repair, leading to arterial narrowing. However, the contributory mechanisms remain poorly understood. Here we show that Krüppel-like factor …
Read PDFThe molecular basis of spermatogenesis, which is a tightly regulated spatiotemporal process in testicular seminiferous tubules where germ cell differentiation and somatic-germ cell interactions drive sperm production, remains incompletely understood. Histological staining techniques lack molecular …
Read PDFColorectal cancer (CRC) is a biologically heterogeneous disease in which single-omics analyses incompletely capture the cross-layer mechanisms underlying tumor progression, immune evasion, and therapeutic resistance. This review critically examines how the integration of genomics, transcriptomics, …
Read PDF