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Semi-supervised Bayesian integration of multiple spatial proteomics datasets
The subcellular localisation of proteins is a key determinant of their function. High-throughput analyses of these localisations can be performed using mass spectrometry-based spatial proteomics, which enables us to examine the localisation and relocalisation of …
Read PDFProspective ICH Q2(R2)-Aligned Total-Error Validation of Label-Free Untargeted Proteomics for Host Cell Protein Quantification in Biotherapeutics
<b>Background</b>: Untargeted proteomics enables quantitative host cell protein (HCP) determination in biotherapeutics, yet no workflow has been validated under ICH Q2(R2) for regulated quality control. <b>Methods</b>: A prospective total-error (TE) validation of label-free ddaPASEF proteomics …
Read PDFProteomics and Aging: Insights From the Osteoporotic Fractures in Men Study
No abstract available.
Read PDFBaseline Immune Signatures in Serum Extracellular Vesicles Distinguish Food-Induced from Wheat-Dependent Exercise-Induced Anaphylaxis
Food-induced anaphylaxis (FIA) is a life-threatening allergic reaction, while wheat-dependent exercise-induced anaphylaxis (WDEIA) is triggered by wheat ingestion plus cofactors. To elucidate their differences, we profiled serum extracellular vesicle (EV) proteomes from 240 participants, including …
Read PDFDistinct Extracellular Matrix Protein Signatures of Cortical and Cancellous Bone Allografts Following Processing for Clinical Use
Demineralized bone matrices (DBMs) are widely used in bone replacement therapy. Bone tissue of either cancellous or cortical origin is decellularized, demineralized, and sterilized during processing, while retaining portions of native organic extracellular matrix (ECM) …
Read PDFDynamic Proximity Networks of Myosin-19 (Myo19) and its Mitochondrial Receptors Miro2 and Metaxin-3
Myosin-19 (Myo19) plays a crucial role in mitochondrial dynamics, cristae organization and ER-mitochondria contact sites. It regulates cytokinesis and inheritance of mitochondria to daughter cells. To better understand the dynamic molecular network of Myo19 during …
Read PDFPlasma small-extracellular vesicles' proteomic signature in neoadjuvant chemotherapy-naïve breast cancer patients
Breast cancer remains a leading cause of cancer-related mortality worldwide, with obesity markedly increasing the risk in affected individuals. Liquid biopsy-based extracellular vesicles (EVs) offer a minimally invasive platform for molecular profiling of tumor-derived markers. …
Read PDFSpecial Issue "Proteomics: An Invaluable Tool to Gain Information on Biological Systems and Their Disease Alterations"
Over the past two decades, proteomics has evolved from a descriptive cataloging of proteins to a sophisticated analytical science that provides deep insights into the dynamic complexity of biological systems [...].
Read PDFAdult patients with autoinflammation of unknown origin partially phenocopy the immune presentation of Still's disease
Autoinflammation of unknown origin remains amongst the most enigmatic of systemic autoinflammatory disorders (SAID), with systemic autoinflammatory symptoms in the absence of a molecular or clinical diagnosis with a recognized SAID. Here, we aim to …
Read PDFLacrimal gland duct obstruction and dacryops: Unravelling its pathogenesis with proteomics and cellular profiling
<h4>Purpose</h4>To understand the pathogenesis of the main lacrimal gland dacryops (ductular cyst) using proteomics and cellular profiling.<h4>Methods</h4>Four patients (mean age, 42.2 ± 18.4 years) with dacryops underwent cyst excision following the aspiration of the cyst …
Read PDFMultimodal Proteomics Reveals Dysregulated Secretion and ECM Remodelling in Schizophrenia Patient iPSC-Derived Astrocytes
Astrocytes are increasingly implicated in the pathophysiology of schizophrenia (SCZ), yet how astrocytic dysfunction contributes to disease-relevant neuronal abnormalities remains unclear. Here, we used mass spectrometry-based proteomics to profile lysates (proteome) and secreted proteins (secretome) …
Read PDFGlobal impact on metabolic capacity of yeast cell factories by optogenetic control of the cAMP-PKA axis
Dynamic metabolic engineering enables temporal redirection of microbial metabolism from biomass production to product synthesis. Here, we show that optogenetic control of protein kinase A (PKA) activity via light-regulated modulation of intracellular cyclic AMP (cAMP) …
Read PDFBody Fluid-based Biomarkers for Cognitive Impairment in Parkinson's Disease
<h4>Background</h4>Parkinson's disease (PD) is the second most common neurodegenerative disorder with cognitive impairment as a prime non-motor symptom. Biomarkers can assist in detecting cognitive impairment, which normally follows movement dysfunction, and also assist in the …
Read PDFMulti-fluid, multi-omics signatures of insulin resistance and incident type 2 diabetes among Puerto Rican adults
<h4>Introduction</h4>Previous studies have examined the prediction of insulin resistance and type 2 diabetes (T2D) using plasma or saliva omics, but none have combined metabolomics and proteomics from multiple biofluids, such as plasma and saliva. Among …
Read PDFOSBPL2 deficiency alleviates diet-induced MASLD by reducing ACSL4-mediated ferroptosis
Oxysterol-binding protein-like 2 (OSBPL2) is a lipid transfer protein that modulates intracellular lipid homeostasis; however, its regulatory role in coordinating iron and fatty acid homeostasis remains largely elusive. A recent study has reported a strong …
Read PDFExploring an Intermediate Colorectal Cancer Screening Test Based on Stool Proteomics and Machine Learning for Optimizing the Selection of Patients for Colonoscopy Identified From FIT
The fecal immunochemical test (FIT) for detecting fecal occult blood, used alone or in combination with other stool biomarkers, has been demonstrated to be effective in the context of colorectal cancer (CRC) screening programs. However, …
Read PDFExploring tear biomarkers with shotgun proteomics for retinoblastoma diagnosis: a pilot study
The most common ocular neoplasia among children is retinoblastoma. Currently, the diagnosis of this disease is essentially clinical, taking a biopsy is contraindicated owing to the high risk of causing metastasis. Therefore, it is imperative …
Read PDFMulti-cohort, cross-species urinary proteomics reveals signatures of LRRK2 dysfunction in Parkinson's disease
Pathogenic mutations in Leucine-rich repeat kinase 2 (LRRK2) are the predominant genetic cause of Parkinson's disease (PD) and often increase kinase activity, making LRRK2 inhibitors promising treatment options. Although LRRK2 kinase inhibitors are advancing clinically, …
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