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Protein structural dynamics drive changes in protein function, making the capture of such dynamics essential for interrogating biological systems. Here we review limited proteolysis coupled to mass spectrometry (LiP-MS), a structural and chemical proteomics method …
Read PDFMultisite chronic pain (MCP) frequently co-occurs with immune and depressive disorders, yet whether it reflects coordinated cross-domain multi-omic dysregulation remains unknown. Using UK Biobank data (19,484 baseline participants; 32,870 to 399,476 for 15.9-year follow-up), we …
Read PDFMany molecular aging biomarkers have been developed to capture heterogeneity in individual aging rates. Yet, systematic comparison of the modeling choices underlying these biomarkers has been limited. In this study, we trained aging biomarkers on …
Read PDFAcute-phase proteins (APPs) are used in veterinary science for diagnosing disease, identifying acute and subclinical inflammatory processes, monitoring disease progression, assessing patient response to treatment, and as a general health screening tool. In turtles, the …
Read PDFTo elucidate the mechanism underlying changes in the water-holding capacity of yak meat during postmortem aging, yak longissimus dorsi muscle was used as the experimental material. Changes in pressure loss, drip loss, cooking loss, and …
Read PDFClinical evidence supports a link between obstructive sleep apnea (OSA) and periodontitis. However, whether OSA exerts a causal effect on periodontitis and the underlying protein biomarkers remains unclear. This study aimed to investigate their causal …
Read PDFThe physiological and social behaviors differ widely between honeybee workers and drones. All the organ rudiments of adult bees are formed during the embryonic stage. The initial molecular bases at the proteomic level for both …
Read PDFWe present a label-free quantitative proteomics dataset from liver tissue of wild-type (WT), heterozygous (HET), and spinal muscular atrophy (SMA) mice at postnatal day 10 (P10). Proteins were extracted using urea lysis, digested with trypsin, …
Read PDFComprehensive target validation remains a significant bottleneck in chemical probe development, particularly for covalent inhibitors, where off-target reactivity can lead to toxicity. Using HMG-CoA synthase 1 (HMGCS1), an underexplored gatekeeper enzyme in the mevalonate pathway, …
Read PDFSpatial context is becoming increasingly important in the omics disciplines. Spatial proteomics is a diverse field encompassing numerous techniques that provide both the location and identity of proteins in biological samples. Improving upon bulk analyses, …
Read PDF<h4>Background</h4>The current method for managing opioid tolerance is opioid rotation, but its effectiveness is limited. Transcutaneous electrical acupoint stimulation (TEAS) combined therapy has been shown to effectively relieve moderate-to-severe pain in patients with hepatocellular carcinoma …
Read PDFShell matrix proteins (SMPs) are key organic components of molluscan biominerals, yet previous nacre proteomics have remained largely qualitative, limiting evaluation of the abundance and fraction association of individual SMPs. Here, we established a quantitative …
Read PDF<h4>Background</h4>Recent advances in proteomic profiling have enabled its use as a powerful approach in elucidating molecular mechanisms underlying Parkinson's disease, enabling the identification of disease-associated protein alterations and candidate biomarkers for diagnosis, progression, and therapeutic …
Read PDFCardiovascular disease (CVD) is the leading cause of morbidity and mortality in Type 1 Diabetes (T1D), but a subset of individuals remains free from macrovascular or renal complications despite decades of hyperglycaemia and a significant …
Read PDF<h4>Objectives</h4>To identify disease-relevant pathways and biomarkers in degenerative cervical myelopathy (DCM) patients from the MYelopathy Natural History Registry.<h4>Methods</h4>Shotgun bottom-up proteomics (DCM <i>n</i> = 20; controls <i>n</i> = 20) was performed using LC-MS/MS in DDA mode. …
Read PDF<h4>Background</h4>Valvular heart disease, particularly aortic valve disease including stenosis and regurgitation, is a common heart disease. This study aimed to explore the protein profiling and the biomarkers in severe aortic valve disease and to provide …
Read PDFAlzheimer's disease (AD) is increasingly recognized as a biological continuum characterized by early neuropathological and molecular changes that precede the onset of clinical symptoms. Fluid biomarkers have transformed the diagnostic landscape by enabling the in …
Read PDFThe blood-brain barrier (BBB), formed primarily by specialized brain endothelial cells (BEC), is essential for nutrient transport, signal transduction, immune cell migration, and pathogen restriction. Although these functions are influenced by the identity and abundance …
Read PDFPersistent symptoms following SARS-CoV-2 infection are the hallmark of post-COVID condition (PCC), also referred to as long COVID. However, the underlying molecular mechanisms remain poorly understood. In this study, we employed data-independent acquisition mass spectrometry …
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