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Sepsis remains a leading cause of pediatric morbidity and mortality, yet its molecular underpinnings are poorly understood. Here, we performed mass spectrometry-based plasma proteomics and cytokine profiling in pediatric sepsis patients at the acute phase …
Read PDFEnterohemorrhagic <i>Escherichia coli</i> O157:H7 (EHEC) is a low-infectious-dose foodborne pathogen that uses norepinephrine gradients in the gut to locate sites for colonization and activate expression of virulence factors. Here, we identified features of transfer RNA …
Read PDFIn cyanobacteria, the redox-sensitive protein OpcA acts as a metabolic switch for G6PDH, enabling rapid adjustment of reducing power generation from glycogen catabolism and thereby precisely regulating carbon flux between anabolic and catabolic pathways. Although …
Read PDFOver the past two decades, molecular biology has witnessed unprecedented innovation driven by the emergence of omics technologies [...].
Read PDFPreeclampsia, a severe pregnancy-induced disorder unique to humans, affects ∼2%-8% of pregnancies globally. Strong evidence supports placental dysfunction as central to preeclampsia; however, there is inadequate understanding of the precise pathogenesis of preeclampsia. In this …
Read PDFNSCLC remains a leading cause of cancer-related mortality, with limited biomarkers to guide surgical and immunotherapeutic intervention. This study leveraged two complementary plasma proteomics platforms, SomaScan (7596 proteins) and NULISA (250 inflammation-related proteins) to profile …
Read PDFNitramines [R(R')N-NO<sub>2</sub>; R,R'=H or alkyl] are valuable synthetic products, but knowledge of the biosynthetic processes that generate these compounds is limited. This work sought to elucidate the biosynthesis of a nitramine natural product, <i>N</i>-nitroglycine (NNG) …
Read PDF<h4>Purpose</h4>Chronic dacryocystitis, characterized by epiphora, is primarily treated with dacryocystorhinostomy (DCR), yet postoperative anastomotic re-obstruction remains a significant cause of failure. This study aimed to identify the key molecular mechanisms driving this recurrence.<h4>Methods</h4>Nasal mucosal tissues …
Read PDFCharacterization of large molecular machines, such as the ribosome, under a breadth of biological states to elucidate regulatory details remains challenging. Herein, a workflow combining complex-up native mass spectrometry (nMS) with infrared multiphoton dissociation (IRMPD) …
Read PDFMedication-related osteonecrosis of the jaw (MRONJ) is a complex condition associated with the use of antiresorptive drugs, such as bisphosphonates and denosumab. The condition is characterized by the presence of exposed bone in the maxillofacial …
Read PDFINTRODUCTION: Polycystic ovary syndrome (PCOS) appears to be closely correlated with obesity; however, no studies have compared the difference between PC-Ob (PCOS plus obesity), obesity-only, and PCOS-only patients at a multi-omics level. In the present …
Read PDF<h4>Background</h4>Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies due to a lack of reliable tools for individualized risk stratification. A comprehensive understanding of the multi-omics landscape may uncover clinically applicable biomarkers and …
Read PDF<h4>Introduction</h4>Current multiple myeloma (MM) risk stratification, anchored on the Revised International Staging System (R-ISS), provides a static snapshot of disease but fails to capture its dynamic biological evolution, functional tumor-microenvironment crosstalk, and real-time treatment response. …
Read PDF<h4>Background</h4>Blood-based biomarkers for stroke subtyping could improve triage in emergency settings. We used cross-platform proteomics to identify plasma biomarkers differentiating major stroke diagnostic groups.<h4>Methods</h4>We conducted a case-control study using 2 biorepositories. Plasma was collected in …
Read PDFCaloric restriction (CR) extends lifespan across diverse organisms, but the effects of CR on human aging and on healthspan are only beginning to be uncovered. In this study, we applied proteomics to plasma samples collected …
Read PDF<h4>Background</h4>Mass spectrometry-based proteomics enables high-throughput quantification of thousands of proteins in clinical samples, fueling biomarker discovery for disease diagnosis and prognosis. However, leveraging complex proteomic profiles for predictive modeling often requires advanced machine learning (ML) …
Read PDFOxidative stress triggers redox-sensitive post-translational modifications, notably disulfide bond formation involving cysteine residues. However, these bonds are often overlooked in proteomics due to the routine use of reducing agents. Here, we employed (LC-MS based metabolomics …
Read PDF<h4>Background</h4>Covalent protein modification by drugs or their reactive metabolites has emerged as an important mechanism underlying pharmacological activity. However, its contribution to the therapeutic effects of compounds derived from traditional Chinese medicine remains insufficiently characterized. …
Read PDFSurvival after lung transplantation lags that of other solid organ transplants. Long-term survival is hampered primarily due to chronic lung allograft dysfunction (CLAD) development. It remains elusive how (chronic) rejection is organized within the lung …
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