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Using fish models to understand the role of aryl hydrocarbon receptor (AHR)-interacting protein (AIP) in controlling sensitivity and resistance to dioxin-like compounds in vivo
Humans are exposed to chemicals such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs) that cause toxicity through activation of the aryl hydrocarbon receptor (AHR). There is inter-individual variation in sensitivity to …
Read PDFEneA of Aspergillus fumigatus is a regulator of secondary metabolism and enhances nscA expression in presence of polyenes and Streptomyces
Aspergillus fumigatus is a soil born fungus, which can induce severe aspergillosis in immunocompromised patients. In this study, we investigated the zinc cluster transcription factor EneA (enhancer of nscA expression A) of A. fumigatus, which …
Read PDFIdentification of ceRNA Regulatory Networks Driven by the lncRNA NEAT1 in Multiple Myeloma
The lncRNA NEAT1 is overexpressed in multiple myeloma (MM) plasma cells and plays a key role in MM pathogenesis. NEAT1 is involved in ceRNA network in several cancers; however, data in MM are virtually absent. …
Read PDFPHYCUT: Scalable Multiplex CRISPR/Cas9 Editing for Genome Engineering in the Diatom <i>Phaeodactylum tricornutum</i>
Diatoms are globally significant microalgae that contribute ∼20% of oxygen production and exhibit remarkable metabolic diversity. The marine diatom <i>Phaeodactylum tricornutum</i> has emerged as a promising synthetic biology platform for the bioproduction of recombinant proteins, …
Read PDFAromatic lactic acid production by <i>Bifidobacterium longum</i> subsp. <i>infantis</i> is determined by cell density, substrate availability, and pH <i>in vitro</i>
<i>Bifidobacterium longum</i> subsp. <i>infantis (B. infantis</i>) is an important colonizer of the infant gut. This species is known to produce a variety of health-beneficial metabolites, including aromatic lactic acids (ALAs), supporting early-life immune development. However, …
Read PDFNatural antisense transcript Nat9a suppresses Scn9a (Na<sub>V</sub>1.7) expression in parvalbumin-positive proprioceptive and inhibitory neurons
Natural antisense transcripts (NATs) are important spatial and temporal regulators of gene expression. We previously cloned a NAT (Nat9a) to Scn9a, which encodes the Na<sub>V</sub>1.7 voltage-gated sodium channel that is essential for pain perception. By …
Read PDFNo Correlation Between Interferon Signaling and Cytosolic Mitochondrial DNA/RNA Leakage in Cultured Skin Fibroblasts of Patients With Mitochondrial Diseases
Mitochondria have long been known to be involved in the regulation of innate immune response. We questioned whether cultured skin fibroblasts of patients suffering from mitochondrial diseases are valuable biological resources for the study of …
Read PDFNorroa™ as a dsRNA biopesticide for <i>Varroa destructor</i>: insights from New Zealand research on mode of action, field efficacy, non-target effects, and social acceptance
RNA interference (RNAi) is emerging as a highly selective approach for pest management, offering species-specific control with limited non-target impacts. In apiculture, dsRNA-based biopesticides represent a novel tool for managing <i>Varroa destructor</i> (hereafter varroa), a …
Read PDFPhotochemical modification of two fluorene-based molecules with DNA intercalating and anti-methicillin resistant Staphylococcus aureus activity
Staphylococcus aureus is a leading cause of skin and soft tissue infections, endocarditis, and bloodstream infections worldwide. The emergence of methicillin-resistant S. aureus (MRSA) and growing resistance to last-resort antibiotics like vancomycin have created an …
Read PDFKLF2 controls thymic epithelial cell homeostasis, impacting regulatory T cell development and immune tolerance
T cell development depends on specialized thymic microenvironments formed by cortical (c) and medullary (m) thymic epithelial cells (TECs), which arise from a common epithelial progenitor. However, the molecular mechanisms that govern TEC differentiation and …
Read PDFCircadian Controlled Transcription in Brain and Peripheral Organs of Juvenile and Adult Mice
Circadian clocks generate daily rhythms of gene expression that influence physiology, disease, and responses to therapeutics, yet how circadian transcription differs between juvenile and adult organisms remains unresolved. Here, we used genome-wide eXcision Repair sequencing …
Read PDFDynamic allele usage of X-linked genes ameliorates neurodevelopmental disease phenotypes in brain organoids
While random X-chromosome inactivation in female cells of placental mammals silences one allele of the majority of X-chromosomal genes, a considerable fraction is only incompletely and variably inactivated. Human model systems to study the dynamics …
Read PDFTYRO3, AXL, MERTK and Their Ligands in Brain Metastases From Colorectal Cancers
<h4>Introduction</h4>TYRO3, AXL, and MERTK (TAM receptor tyrosine kinases) represent potential therapeutic targets in metastatic colorectal cancer. Pre-clinical and clinical data are needed to explore further how TAM receptors interact with the central nervous system, which …
Read PDFOmics-aided design genome editing strategy for challenging human immortalized cell models
CRISPR-Cas9 has become a popular genome editing tool for biomedical research and drug development due to its capability to enable precise correction or integration of genetic mutations in the genome. However, precise genome editing competency …
Read PDFNRF2 controls a diverse network of antiviral effectors with p62 acting as a central restriction factor effective across virus families
The transcription factor erythroid 2 (NFE2)-related factor 2 (NRF2) is a key regulator of cellular homeostasis. Recent discoveries have identified agonists of NRF2 as inducers of broad cellular resistance to viral infection including SARS-CoV-2. Nevertheless, …
Read PDFArp2/3 complex and β1 integrin drive an invasive front through extracellular matrix adaptation in pancreatic cancer
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest malignancies, due to its aggressive invasiveness and resistance to therapy. The dense, stiff extracellular matrix, composed primarily of collagen I and basement membrane components such as …
Read PDFEpstein-Barr virus-transformed B-cells from a hypoxia model of the germinal center requires external unsaturated fatty acids
Epstein-Barr virus (EBV) drives over 200,000 cancer cases annually, including diffuse large B-cell lymphoma, Burkitt lymphoma, and classic Hodgkin lymphoma-malignancies that frequently originate from germinal centers (GCs), which are physiologically hypoxic (O2 < 1%). However, …
Read PDFThe <i>Bitter Taste Receptor (T2R)</i> Gene Repertoire in the Porcine Circumvallate Papillae Consists of Fourteen Genes, Including Two Newly Validated <i>T2R61</i> and <i>T2R62</i>
<h4>Background/objectives</h4>Bitter taste perception is important for pig feeding behavior and survival. The type 2 taste receptors (T2Rs) are G protein-coupled receptors responsible for the sense of bitter taste perception in mammals. <i>T2Rs</i> are expressed in …
Read PDFA scalable, multi-resolution consensus clustering approach for prioritizing robust signals from high-throughput screens
Modern biology increasingly relies on large-scale screening to generate high dimensional datasets with potential to accelerate discovery. However, analysing these complex datasets remains challenging, due to hierarchical biological structure, uncertainty in the true number of …
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