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OBJECTIVES: The gaur (Bos gaurus) is one of the largest wild cattle species in the Bovidae family and is among the most threatened mammals in Vietnam. Listed as vulnerable on the IUCN Red List since …
Read PDFWe report a near-complete genome sequence of <i>Mycoplasma meleagridis</i> isolated from a turkey in New Zealand. Using long-read sequencing approaches, a single circular scaffold was assembled, and then annotated. The genome reported here is the …
Read PDF<i>Pediococcus pentosaceus</i> TOKAI 10 m, isolated from Japanese Takuan pickle, shows potential as a soy milk yogurt starter. Its genome comprises a 1,788,781 bp chromosome and four plasmids (56,333, 13,649, 10,458, and 10,048 bp), with …
Read PDFDNA has been proposed as an alternative to magnetic and solid-state devices for storing digital data. In DNA data storage, writing data is performed through DNA synthesis, and reading is done via sequencing. Nanopore devices …
Read PDFThe gold standard for bloodstream infection (BSI) diagnostics involves culturing positive blood cultures (BCs) using phenotypic methods for organism identification and antimicrobial resistance (AMR) testing, which can take up to five days. However, it is …
Read PDF<h4>Background</h4><i>Pasteurella multocida</i> is a Gram-negative bacterium responsible for various diseases in animals, including hemorrhagic septicemia (HS). Oxford Nanopore Technologies' (ONT) platform streams the sequencing data immediately, allowing for on-the-fly analysis. This capability enables researchers to …
Read PDFWe present a genome of Echinomermella matsi (Nematoda: Plectida: Benthimermithidae), a body cavity parasite of the green sea urchin Strongylocentrotus spp. commonly found along the coast of Central and Northern Norway. Three assemblies were generated, …
Read PDFWe report a chromosome-level assembly of a male red coachwhip snake (<i>Masticophis flagellum piceus</i>) generated exclusively with nanopore sequencing. Using Hifiasm-ONT for assembly and RagTag for scaffold polishing, we produced a 1.61 Gb nuclear genome …
Read PDFAs sequencing costs decrease, short-read and long-read technologies are indispensable tools for uncovering the genetic drivers behind bacterial pathogen resistance. This study explores the differences between the use of short-read (Illumina) and long-read (Oxford Nanopore …
Read PDFField-deployable DNA metabarcoding offers a transformative approach to biodiversity research and monitoring, yet its application remains limited due to technical constraints and a lack of reference data in poorly studied ecosystems. Combining isothermal Recombinase Polymerase …
Read PDFDengue virus (DENV) poses a growing risk in Tanzania, yet its genetic diversity in mosquito populations remains poorly understood. Using Nanopore sequencing, we recovered full coding sequences from six DENV-2 positive mosquito pools collected in …
Read PDFTomato yellow leaf curl disease is a major viral disease severely affecting tomato crops in the Mediterranean region, leading to reduced crop yield and significant economic losses. The disease is caused by monopartite begomoviruses belonging …
Read PDFThe complete genome sequence of <i>Diaporthe vaccinii</i> Shear was reported using combined Oxford Nanopore and Illumina sequencing. The hybrid genome comprises 64 contigs with a total length of 60.2 Mb and a GC content of …
Read PDF<i>Actinomyces oris</i> MG-1 is a key oral commensal involved in dental plaque formation. We report its complete genome sequence, generated using Illumina and Oxford Nanopore sequencing. The genome consists of a single circular chromosome of …
Read PDF<b>Background.</b> Our recent findings indicate that the rough form of the <i>Mycobacterium abscessus</i> complex (MABC) is associated with worse clinical outcomes compared to the smooth variant. The glycopeptidolipid (GPL) locus has been considered vital for …
Read PDFThis study combines Oxford Nanopore (ONT) third-generation sequencing with targeted amino acid metabolomics to elucidate the mechanisms underlying the structural and metabolic responses of the microbial community in Golden Ear Mushroom (<i>Naematelia sinensis</i>) during <i>Trichoderma</i> …
Read PDFIdentification of genomic rearrangements by microarrays or short-read sequencing frequently lacks information about the exact architecture and breakpoints of variants due to technical limitations. Independent verification of complex structural variants (SVs) is often performed using …
Read PDFShort tandem repeats (STRs) are highly polymorphic repetitive DNA sequences extensively used in forensic science for identification of individuals. STR genotyping is usually performed by capillary electrophoresis (CE) or next-generation sequencing (NGS) in centralized laboratories. …
Read PDF<i>Enterococcus faecium</i> strain AJ_C_05 was isolated from a Deonjang, a traditional Korean fermented food made from soybeans, and its complete genome was sequenced using the Oxford MinION platform. The complete genome of <i>E. faecium</i> strain …
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