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<h4>Background</h4>Advances in our technological capacity to interrogate CNS tumor biology have led to the ever increasing use of genomic sequencing in diagnostic decision making. Presently, CNS tumors are classified based on their epigenetic signatures, leading …
Read PDFRibosomes are key molecular machines that translate mRNA into proteins. Mitoribosomes are specific ribosomes found in mitochondria, which have been shown to be remarkably diverse across eukaryotic lineages. In plants, they possess unique features, including …
Read PDFFreshwater ecosystems are vital for biodiversity and livelihoods in Bangladesh, where interest in fish gut microbiota is growing to aid aquaculture sustainability through microbial interventions. Therefore, this research investigated the bacteriomes of the gut of …
Read PDF<h4>Background</h4>DNA methylation is an epigenetic mechanism involved in gene regulation and cellular differentiation. Accurate and comprehensive assessment of DNA methylation patterns is thus essential for understanding their role in various biological processes and disease mechanisms. …
Read PDFCLN2 disease (neuronal ceroid lipofuscinosis type 2) is an ultra-rare lysosomal storage disorder caused by mutations in the TPP1/CLN2 gene, resulting in impaired tripeptidyl peptidase 1 (TPP1) activity. The timely initiation of enzyme replacement therapy …
Read PDFMosquito-borne diseases are responsible for over 600,000 deaths annually, mainly in sub-Saharan countries. Mosquito surveillance is a crucial element of vector control programmes to assure they remain effective. This study focused on optimizing the use …
Read PDF<h4>Background</h4>Genomics is critical for malaria control and elimination by providing population-level monitoring of malarial gene flow. Whole genome sequencing is valuable for identifying such genomic changes and antimalarial drug resistance. However, sequencing submicroscopic and asymptomatic …
Read PDFRapid, accurate and scalable sequencing of bacteriophage genomes is critical to advance phage therapy, build phage biobanks and understand phage genomic diversity. Current methods are based on sequencing and assembling complete bacteriophage genomes using short- …
Read PDFOxford Nanopore Technologies (ONT) offers an efficient and effective solution for studying DNA methylation. Both R9.4.1 and R10.4.1 ONT chemistries have been widely used to generate numerous DNA methylation data, making it inevitable to conduct …
Read PDFZancudomyces culisetae is an obligate symbiotic fungus inhabiting the digestive tracts of aquatic insect larvae, including black flies, midges, and mosquitoes. With a global distribution and high prevalence in disease-transmitting insects, Z. culisetae serves as …
Read PDFCore-genome multi-locus sequence typing (cgMLST) is a well-established and standardized method for genomics-based cluster detection and phylogenomic analysis of bacteria. The reduced error rate of Oxford Nanopore Technologies (ONT) R10 sequencing has prompted many laboratories …
Read PDFRespiratory infections pose a significant risk for people requiring prolonged mechanical ventilation, yet limited information exists regarding the complex microbiome dynamics of people with tracheostomies during chronic critical illness. Oxford Nanopore Technologies (ONT) long-read sequencing …
Read PDF<h4>Objectives</h4>We describe the identification and characterization of the HMB-3 variant, produced by a P. asiatica isolate from a patient in Switzerland.<h4>Materials and methods</h4>A carbapenem-resistant P. asiatica isolate was sent to the Swiss National Reference Center …
Read PDFThalassemia is a hereditary hemolytic disorder primarily caused by defects in the hemoglobin genes, which impede the synthesis of hemoglobin peptide chains. This disease is mainly classified into two types: α and β. Currently, there …
Read PDFBACKGROUND: Amplicon sequencing of Mycobacterium tuberculosis resistance-associated genes offers a cost-effective alternative to whole-genome sequencing for rapid profiling of infections and guiding clinical management. However, existing assays require frequent manual updates to accommodate emerging resistance …
Read PDFWe report a leg ulcer infection caused by <i>Vibrio metschnikovii</i> in a patient with chronic venous leg ulcers in Montevideo, Uruguay. Shotgun MS/MS combined with long-read whole-genome sequencing confirmed species assignment to <i>V. metschnikovii</i>. In …
Read PDF<i>Acinetobacter baumannii</i> is a leading cause of nosocomial infections and ranks first on the World Health Organization's list of critical-priority pathogens for antimicrobial resistance, surveillance, and new drug development. We report the whole-genome assembly of …
Read PDFLong-read sequencing has transformed many areas of biology and holds significant promise for telomere research by enabling analysis of nucleotide-level resolution chromosome arm-specific telomere length in both model organisms and humans. However, the adoption of …
Read PDF<h4>Background</h4>The Purpureocillium lilacinum NCHU-NPUST-175 (Pl-NCHU-NPUST-175) strain has been previously reported as an entomopathogenic fungus (EPF) with potential for controlling Forcipomyia taiwana (little black mosquito). In this study, genome sequencing of Pl-NCHU-NPUST-175 was performed via long-read …
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