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Rhizoctonia solani is a ubiquitously distributed soil-borne fungal pathogen that causes serious diseases in many plants worldwide. It attracts significant research attention due to its considerable economic importance in agricultural production. However, the limited availability …
Read PDFWe present the first reference genome of the highly heterozygous autotetraploid Allium porrum (leek). Combining long-read sequencing with single-nucleotide polymorphism (SNP)-array screening of two experimental F1 populations, we generated a genetic map with 11,429 SNP …
Read PDF<h4>Background</h4>Understanding the structure and function of microbial genomes is crucial for uncovering their ecological roles, evolutionary trajectories, and potential applications in health, biotechnology, agriculture, food production, and environmental science. However, genome reconstruction and annotation remain …
Read PDFThe genus Stichopus comprises sea cucumber species that are ecologically and economically valuable, known for their roles in nutrient cycling and as sources of bioactive compounds in traditional medicine. Genomic insights are essential for understanding …
Read PDFArgan (Sideroxylon spinosum L., formerly Argania spinosa) is a tree endemic to Morocco, primarily valued for its seed oil. Growing interest in its biology and in genes linked to oil quality and stress resistance highlights …
Read PDFSugar beet (<i>Beta vulgaris</i> ssp. <i>vulgaris</i>) is an important crop plant serving as a major source of sugar, particularly in Europe. Sugar beet research using the genotype KWS2320 has a long-standing history, and many datasets …
Read PDFGenetic maps are a key resource for the analysis of genetic variation. Prior maps in dogs were based on the canFam3.1 genome assembly, which contains thousands of sequence gaps that include functional elements. Although long-read …
Read PDFBelamcanda chinensis is a non-leguminous medicinal plant rich in bioactive isoflavones; however, the lack of a high-quality reference genome has limited elucidation of its isoflavone biosynthetic and modification network. Here, we present the first near …
Read PDFThe fruit fly Drosophila melanogaster is a pivotal model organism, yet its reference genome (ISO-1 strain) retains unresolved gaps in complex regions. Here, we present a near-complete telomere-to-telomere genome assembly (Dm.nT2T) of the Canton S …
Read PDFPolycyclic aromatic hydrocarbons (PAHs) are persistent environmental pollutants with significant ecological and health risks. Among them, coronene, a high molecular weight PAH, is particularly resistant to biodegradation due to its complex structure. This study characterizes …
Read PDFThe wasp Vespa mandarinia raised in Yunnan Province of China exhibits a relatively large body size, potent toxicity, invasive potential, and ecological impacts. These characteristics are closely linked to its acute olfactory sensitivity, venom-aided predation, …
Read PDFElaeocarpus petiolatus is an ecologically and economically important species in tropical and subtropical forests. Despite its significance, the lack of genomic resources has hindered research on the genetic diversity and adaptive traits of E. petiolatus. …
Read PDFTea plants possess a highly heterozygous genome and produce diverse beneficial metabolites, yet the genomic basis of its metabolic diversity remains fully elusive. Here, we show that single-cell sequencing of 107 sperm cells, combined with …
Read PDFOnthophagus binodis is a coprophagous scarab beetle native to southern Africa. This species and many others in the tribe Onthophagini have been introduced to farms across multiple continents in the context of cattle pasture management …
Read PDFThe bumblebee Bombus patagiatus Nylander, 1848 and Bombus lantschouensis Vogt, 1908 (Hymenoptera: Apidae) are ecologically important bumble bee species native to East Asia, with considerable value for agricultural pollination and domestication. Despite their ecological and …
Read PDFCamellia pitardii Cohen-Stuart, is an endemic winter-spring flowering wild Camellia species in southwestern China, with important ecological and horticultural value. Here, we report the near telomere-to-telomere (T2T) genome of C. pitardii by combining PacBio HiFi, …
Read PDFBitter gourd (Momordica charantia) is an economically important vegetable crop with notable medicinal properties, yet its genetic improvement has been hindered by limited genomic resources and insufficient functional genomics tools. Here, we present a comprehensive …
Read PDFThe spatial organization of adaptive immune cells within lymph nodes is critical for understanding immune responses during infection and disease. Here, we introduce AIR-SPACE, an integrative approach that combines high-resolution spatial transcriptomics with paired, high-fidelity …
Read PDF<b>Background/Objectives:</b> The vaginal microbiota constitutes a highly dynamic microbial ecosystem shaped by the distinct mucosal, hormonal, and immunological environment of the female genital tract. Accumulating evidence suggests that shifts in cervical microbial composition and function …
Read PDFEfficient DNA extraction from coastal sediments is a critical step for studying microbial communities, especially when targeting high-molecular-weight DNA for long-read sequencing. Here, we compare a cost-effective laboratory-made (LM) extraction method with two widely used …
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