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1474 results

Full text 2026

Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries

Veseljak D, Despot-Slade E, Volarić M, et al.

<h4>Background</h4>Tandemly repeated satellite DNAs (satDNAs) are among the most copious sequences of eukaryotic genomes. They often reside in centromeric regions, but their diversity among different organisms obscures the properties that centromere-competent satDNAs should possess.<h4>Results</h4>Here, we …

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Epigenetics Genome Assembly Nanopore Sequencing
Full text 2025

Nuclear and organelle genome assemblies of 5 Cucumis melo L. accessions, Ananas, Canton, PI 414723, Vedrantais, and Zhimali, belonging to diverse botanical groups

Belinchon-Moreno J, Berard A, Canaguier A, et al.

The construction of accurate whole genome sequences is pivotal for characterizing the genetic diversity of plant species, identifying genes controlling important traits, or understanding their evolutionary dynamics. Here, we generated the nuclear, mitochondrial, and chloroplast …

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Nanopore Sequencing
Full text 2025

Chromosome-level genome assembly of Hippophae salicifolia

Zeng Z, Mao C, Zhang J, et al.

Hippophae salicifolia, a dioecious small tree species endemic to the Himalayan region, holds great potential in both ecological conservation and industrial applications. In this study, we employed PacBio HiFi long reads, Illumina short reads, and …

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Genome Assembly Nanopore Sequencing
Full text 2025

Reference-based chromosome-scale assembly of Japanese barley (Hordeum vulgare ssp. vulgare) cultivar Hayakiso 2

Tanaka T, Haraguchi Y, Todoroki T, et al.

Current advances in next-generation sequencing (NGS) technology and assembling programs permit construct chromosome-level genome assemblies in various plants. In contrast to resequencing, the genome sequences provide comprehensive annotation data useful for plant genetics and breeding. …

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Genome Assembly Nanopore Sequencing
Full text 2025

Beyond single references: pangenome graphs and the future of genomic medicine

Nyaga DM, Zaied RE, Silander OK, et al.

Genomic medicine relies on single reference genomes that miss crucial genetic diversity, creating diagnostic gaps that disproportionately affect underrepresented populations. Pangenome graphs, collections of diverse genomes represented as interconnected genetic paths, offer a powerful alternative …

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Genome Assembly Nanopore Sequencing Structural Variants
Full text 2025

Haplotype-resolved genome assembly of the tetraploid potato cultivar Désirée

Godec T, Beier S, Rodriguez-Granados NY, et al.

Cultivar Désirée is an important model for potato functional genomics studies to assist breeding strategies. Here, we present a haplotype-resolved genome assembly of Désirée, achieved by assembling PacBio HiFi reads and Hi-C scaffolding, resulting in …

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Nanopore Sequencing
Full text 2025

Colora: a Snakemake workflow for complete chromosome-scale de novo genome assembly

Obinu L, Booth T, De Weerd H, et al.

<h4>Motivation</h4>De novo assembly creates reference genomes that underpin many modern biodiversity and conservation studies. Large numbers of new genomes are being assembled by labs around the world. To avoid duplication of efforts and variable data …

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Genome Assembly Nanopore Sequencing Workflows & Pipelines